Physiochemical changes mediated by “Candidatus Liberibacter asiaticus” in Asian citrus psyllids

Banafsheh Molki; Phuc Thi Ha; Abdelrhman Mohamed; Nabil Killiny; David R Gang; Anders Omsland; Haluk Beyenal

doi:10.1038/s41598-019-52692-7

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Physiochemical changes mediated by “Candidatus Liberibacter asiaticus” in Asian citrus psyllids

Journal article

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Physiochemical changes mediated by “Candidatus Liberibacter asiaticus” in Asian citrus psyllids

Banafsheh Molki, Phuc Thi Ha, Abdelrhman Mohamed, Nabil Killiny, David R Gang, Anders Omsland and Haluk Beyenal

Scientific reports, Vol.9(1), pp.16375-9

11/08/2019

DOI: https://doi.org/10.1038/s41598-019-52692-7

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https://hdl.handle.net/2376/102079

PMCID: PMC6841951

PMID: 31704963

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Abstract

Biotechnology

Biological techniques

Plant pathogenic bacteria interact with their insect host(s)/vector(s) at the cellular and molecular levels. This interaction may alter the physiology of their insect vector, which may also promote the growth and transmission of the bacterium. Here we studied the effect of “ Candidatus Liberibacter asiaticus” (“ Ca . L. asiaticus”) on physiochemical conditions within its insect vector, the Asian citrus psyllid (ACP), and whether these changes were beneficial for the pathogen. The local microenvironments inside ACPs were quantified using microelectrodes. The average hemolymph pH was significantly higher in infected ACPs (8.13 ± 0.21) than in “ Ca . L. asiaticus”-free ACPs (7.29 ± 0.15). The average hemolymph oxygen tension was higher in “ Ca . L. asiaticus”-free ACPs than in infected ACPs (67.13% ± 2.11% vs. 35.61% ± 1.26%). Oxygen tension reduction and pH increase were accompanied by “ Ca . L. asiaticus” infection. Thus, oxygen tension of the hemolymph is an indicator of infection status, with pH affected by the severity of the infection.

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Title: Physiochemical changes mediated by “Candidatus Liberibacter asiaticus” in Asian citrus psyllids
Creators: Banafsheh Molki - Washington, USA
Phuc Thi Ha - Washington, USA
Abdelrhman Mohamed - Washington, USA
Nabil Killiny - Lake Alfred, Florida USA
David R Gang - Washington, USA
Anders Omsland - Washington, USA
Haluk Beyenal - Washington, USA
Publication Details: Scientific reports, Vol.9(1), pp.16375-9
Academic Unit: Paul G. Allen School for Global Animal Health; Chemical Engineering and Bioengineering, School of; Biological Chemistry, Institute of
Publisher: Nature Publishing Group UK; London
Grant note: 2016-70016-24824 / ;
Identifiers: 99900546516701842
Language: English
Resource Type: Journal article