Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation

Chao Wang; Haixiang Yu; Zhongming Zhang; Liangliang Yu; Zonglie Hong; Li Luo; Xiaoshu Xu

doi:10.1094/MPMI-02-15-0032-R

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Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation

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Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation

Chao Wang, Haixiang Yu, Zhongming Zhang, Liangliang Yu, Zonglie Hong, Li Luo and Xiaoshu Xu

Molecular plant-microbe interactions, Vol.28(8), pp.847-855

08/2015

DOI: https://doi.org/10.1094/MPMI-02-15-0032-R

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https://hdl.handle.net/2376/110394

PMID: 25775272

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Abstract

Amino Acid Sequence

Promoter Regions, Genetic

Root Nodules, Plant - microbiology

Molecular Sequence Data

Root Nodules, Plant - genetics

Loteae - microbiology

Cyclopentanes - pharmacology

Peptide Hormones - genetics

Rhizobium - physiology

Arabidopsis - metabolism

Symbiosis - genetics

Arabidopsis - genetics

Sequence Homology, Amino Acid

Oxylipins - metabolism

Plant Proteins - genetics

Gene Expression Regulation, Plant - drug effects

Glucuronidase - genetics

Plants, Genetically Modified

Oxylipins - pharmacology

Cyclopentanes - metabolism

Plant Proteins - metabolism

Root Nodules, Plant - growth & development

Loteae - genetics

Phytosulfokine (PSK) is a tyrosine-sulfated peptide that is widely distributed in plants, participating in cell proliferation, differentiation, and innate immunity. The potential role of PSK in nodulation in legumes has not been reported. In this work, five PSK precursor genes were identified in Lotus japonicas, designated as LjPSK1 to LjPSK5. Three of them (LjPSK1, LjPSK4, and LjPSK5) were found to be expressed in nitrogen-fixing root nodules. LjPSK1 and LjPSK4 were not induced at the early stage of nodulation. Interestingly, while the expression of LjPSK4 was also found in spontaneous nodules without rhizobial colonization, LjPSK1 was not induced in these pseudo nodules. Promoter-β-glucuronidase analysis revealed that LjPSK1 was highly expressed in enlarged symbiotic cells of nodules. Exogenous addition of 1 1M synthetic PSK peptide resulted in increased nodule numbers per plant. Consistently, the number of mature nodules but not the events of rhizobial infection and nodule initiation was increased by overexpressing LjPSK1 in transgenic hairy roots, in which the expression of jasmonate-responsive genes was found to be repressed. These results suggest that PSK is a new peptide signal that regulates nodulation in legumes, probably through cross-talking with other phytohormones.

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Title: Phytosulfokine Is Involved in Positive Regulation of Lotus japonicus Nodulation
Creators: Chao Wang - 3 State Key Lab of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China
Haixiang Yu - 2 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
Zhongming Zhang - Huazhong Agricultural University
Liangliang Yu - 1 Shanghai Key Lab of Bio-energy Crops, School of Life Sciences, Shanghai University, Shanghai, 200444, China
Zonglie Hong - 4 Department of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844, U.S.A
Li Luo - 1 Shanghai Key Lab of Bio-energy Crops, School of Life Sciences, Shanghai University, Shanghai, 200444, China
Xiaoshu Xu - Chinese Academy of Sciences
Publication Details: Molecular plant-microbe interactions, Vol.28(8), pp.847-855
Academic Unit: Center for Reproductive Biology
Publisher: Amer Phytopathological Soc; United States
Number of pages: 9
Grant note: 14JC1402300 / Shanghai Key Projects of Basic Research 2011CB100702 / National Basic Research Program of China 31370277 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 15YF1403800 / Shanghai Sailing Program
Identifiers: 99900547264101842
Language: English
Resource Type: Journal article