Journal article
PiSCP1 and PiCDPK2 Localize to Peroxisomes and Are Involved in Pollen Tube Growth in Petunia Inflata
Plants (Basel), Vol.2(1), pp.72-86
03/01/2013
Handle:
https://hdl.handle.net/2376/100660
PMCID: PMC4844289
PMID: 27137367
Abstract
Petunia inflata
small CDPK-interacting protein 1 (PiSCP1) was identified as a pollen expressed PiCDPK1 interacting protein using the yeast two hybrid system and the interaction confirmed using pull-down and phosphorylation assays. PiSCP1 is pollen specific and shares amino acid homology with uncharacterized proteins from diverse species of higher plants, but no protein of known function. Expression of PiSCP1-GFP
in vivo
inhibited pollen tube growth and was shown to localize to peroxisomes in growing pollen tubes. As PiCDPK1 is plasma membrane localized, we investigated the localization of a second isoform, PiCDPK2, and show that it co-localizes to peroxisomes with PiSCP1 and that the two proteins interact in the yeast 2 hybrid interaction assay, suggesting that interaction with the latter CDPK isoform is likely the one of biological relevance. Both PiCDPK2 and PiSCP1 affect pollen tube growth, presumably by mediating peroxisome function, however how they do so is currently not clear.
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Details
- Title
- PiSCP1 and PiCDPK2 Localize to Peroxisomes and Are Involved in Pollen Tube Growth in Petunia Inflata
- Creators
- Feng Guo - Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA; E-MailGyeong Mee Yoon - Department of Biology University of North Carolina, Chapel Hill, NC 27599, USA; E-MailAndrew G McCubbin - School of Biological Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Plants (Basel), Vol.2(1), pp.72-86
- Academic Unit
- Biological Sciences, School of
- Publisher
- MDPI
- Identifiers
- 99900546615101842
- Language
- English
- Resource Type
- Journal article