Journal article
Platelet proteome changes associated with diabetes and during platelet storage for transfusion
Journal of proteome research, Vol.8(5), pp.2261-2272
05/2009
Handle:
https://hdl.handle.net/2376/107243
PMCID: PMC3017716
PMID: 19267493
Abstract
Human platelets play a key role in homeostasis and thrombosis and have recently emerged as key regulators of inflammation. Platelets stored for transfusion produce pro-thrombotic and pro-inflammatory mediators implicated in adverse transfusion reactions. Correspondingly, these mediators are central players in pathological conditions including cardiovascular disease, the major cause of death in diabetics. In view of this, a mass spectrometry based proteomics study was performed on platelets collected from healthy and type-2 diabetics stored for transfusion. Strikingly, our innovative and sensitive proteomic approach identified 122 proteins that were either up- or down-regulated in type-2 diabetics relative to non-diabetic controls and 117 proteins whose abundances changed during a 5-day storage period. Notably our studies are the first to characterize the proteome of platelets from diabetics before and after storage for transfusion. These identified differences allow us to formulate new hypotheses and experimentation to improve clinical outcomes by targeting “high risk platelets” that render platelet transfusion less effective or even unsafe.
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Details
- Title
- Platelet proteome changes associated with diabetes and during platelet storage for transfusion
- Creators
- David L Springer - Fundamental Science Division, Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland WA 99352, USAJohn H Miller - School of Electrical engineering and Computer Science, Washington State University Tri-Cities, 2710 University Drive, Richland, WA 99352Sherry L Spinelli - Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester NY 14642, USALjiljana Pasa-Tolic - Fundamental Science Division, Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland WA 99352, USASamuel O Purvine - Fundamental Science Division, Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland WA 99352, USADonald S Daly - Fundamental Science Division, Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland WA 99352, USARichard C Zangar - Fundamental Science Division, Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland WA 99352, USAShuangshuang Jin - School of Electrical engineering and Computer Science, Washington State University Tri-Cities, 2710 University Drive, Richland, WA 99352Neil Blumberg - Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester NY 14642, USACharles W Francis - Medicine, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester NY 14642, USAMark B Taubman - Medicine, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester NY 14642, USAAnn E Casey - Environmental Medicine, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester NY 14642, USASteven D Wittlin - Medicine, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester NY 14642, USARichard P Phipps - Pathology, Environmental Medicine, Medicine and Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester NY 14642, USA
- Publication Details
- Journal of proteome research, Vol.8(5), pp.2261-2272
- Academic Unit
- Engineering and Applied Sciences (TRIC), School of
- Identifiers
- 99900547177201842
- Language
- English
- Resource Type
- Journal article