Journal article
Polyamine depletion down-regulates expression of the Trichomonas vaginalis cytotoxic CP65, a 65-kDa cysteine proteinase involved in cellular damage
The international journal of biochemistry & cell biology, Vol.40(11), pp.2442-2451
2008
Handle:
https://hdl.handle.net/2376/114607
PMID: 18586550
Abstract
Recently, we found that inhibition of putrescine synthesis by ornithine decarboxylase (ODC) significantly increased
Trichomonas vaginalis adherence mediated by protein adhesins. Surprisingly and unexpectedly, trichomonal contact-dependent cytotoxicity was absent. Therefore, a role for polyamine depletion on regulation of
T. vaginalis cytotoxicity mediated by the cysteine proteinase (CP) of 65-kDa, CP65, was investigated. We performed cytotoxicity and cell-binding assays followed by zymograms, as well as Western blot and indirect immunofluorescence assays using specific anti-CP65 antibodies to detect CP65. Trichomonads grown in the presence of the ODC inhibitor, 1-4-diamino-2-butanone (DAB) had lower levels of cytotoxicity that corresponded with diminished CP65 proteolytic activity when compared to untreated organisms handled identically. Likewise, semiquantitative and qRT-PCR as well as Western blot and immunofluorescence assays showed decreased amounts of
tvcp65 mRNA and CP65 protein in DAB-treated parasites. These effects were reversed by addition of exogenous putrescine. These data show a direct link between polyamine metabolism and expression of the cytotoxic CP65 proteinase involved in trichomonal host cellular damage.
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Details
- Title
- Polyamine depletion down-regulates expression of the Trichomonas vaginalis cytotoxic CP65, a 65-kDa cysteine proteinase involved in cellular damage
- Creators
- María Elizbeth Alvarez-Sánchez - Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México (UACM), San Lorenzo # 290, Col. Del Valle, CP 03100 Mexico City, MexicoBertha Isabel Carvajal-Gamez - Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México (UACM), San Lorenzo # 290, Col. Del Valle, CP 03100 Mexico City, MexicoEduardo Solano-González - Departamento de Biotecnología y Bioingeniería Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico City, MexicoMáximo Martínez-Benitez - Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México (UACM), San Lorenzo # 290, Col. Del Valle, CP 03100 Mexico City, MexicoAna F Garcia - Department of Microbiology and Immunology, University of Texas Health Science Center, San Antonio, TX, USAJohn F Alderete - School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4660, USARossana Arroyo - Departamento de Patología Experimental, CINVESTAV-IPN, Av IPN 2508, Col. San Pedro Zacatenco, CP 07360 Mexico City, Mexico
- Publication Details
- The international journal of biochemistry & cell biology, Vol.40(11), pp.2442-2451
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547604801842
- Language
- English
- Resource Type
- Journal article