Polycaprolactone-coated 3D printed tricalcium phosphate scaffolds for bone tissue engineering: in vitro alendronate release behavior and local delivery effect on in vivo osteogenesis

Solaiman Tarafder; Susmita Bose

doi:10.1021/am501048n

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Polycaprolactone-coated 3D printed tricalcium phosphate scaffolds for bone tissue engineering: in vitro alendronate release behavior and local delivery effect on in vivo osteogenesis

Journal article

Open access

Peer reviewed

Polycaprolactone-coated 3D printed tricalcium phosphate scaffolds for bone tissue engineering: in vitro alendronate release behavior and local delivery effect on in vivo osteogenesis

Solaiman Tarafder and Susmita Bose

ACS applied materials & interfaces, Vol.6(13), pp.9955-9965

07/09/2014

DOI: https://doi.org/10.1021/am501048n

Handle:

https://hdl.handle.net/2376/108046

PMCID: PMC4095936

PMID: 24826838

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Abstract

Osteogenesis - drug effects

Rats

Bone Density Conservation Agents - administration & dosage

Rats, Sprague-Dawley

Bone Density Conservation Agents - pharmacology

Tissue Scaffolds

Polyesters - chemistry

Animals

Alendronate - pharmacology

In Vitro Techniques

Printing, Three-Dimensional

Alendronate - administration & dosage

Tissue Engineering

The aim of this work was to evaluate the effect of in vitro alendronate (AD) release behavior through polycaprolactone (PCL) coating on in vivo bone formation using PCL-coated 3D printed interconnected porous tricalcium phosphate (TCP) scaffolds. Higher AD and Ca(2+) ion release was observed at lower pH (5.0) than that at higher pH (7.4). AD and Ca(2+) release, surface morphology, and phase analysis after release indicated a matrix degradation dominated AD release caused by TCP dissolution. PCL coating showed its effectiveness for controlled and sustained AD release. Six different scaffold compositions, namely, (i) TCP (bare TCP), (ii) TCP + AD (AD-coated TCP), (iii) TCP + PCL (PCL-coated TCP), (iv) TCP + PCL + AD, (v) TCP + AD + PCL, and (vi) TCP + AD + PCL + AD were tested in the distal femoral defect of Sprague-Dawley rats for 6 and 10 weeks. An excellent bone formation inside the micro and macro pores of the scaffolds was observed from histomorphology. Histomorphometric analysis revealed maximum new bone formation in TCP + AD + PCL scaffolds after 6 weeks. No adverse effect of PCL on bioactivity of TCP and in vivo bone formation was observed. All scaffolds with AD showed higher bone formation and reduced TRAP (tartrate resistant acid phosphatase) positive cells activity compared to bare TCP and TCP coated with only PCL. Bare TCP scaffolds showed the highest TRAP positive cells activity followed by TCP + PCL scaffolds, whereas TCP + AD scaffolds showed the lowest TRAP activity. A higher TRAP positive cells activity was observed in TCP + AD + PCL compared to TCP + AD scaffolds after 6 weeks. Our results show that in vivo local AD delivery from PCL-coated 3DP TCP scaffolds could further induce increased early bone formation.

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Title: Polycaprolactone-coated 3D printed tricalcium phosphate scaffolds for bone tissue engineering: in vitro alendronate release behavior and local delivery effect on in vivo osteogenesis
Creators: Solaiman Tarafder - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University , Pullman, Washington 99164, United States
Susmita Bose
Publication Details: ACS applied materials & interfaces, Vol.6(13), pp.9955-9965
Academic Unit: Mechanical and Materials Engineering, School of
Publisher: United States
Grant note: R01-EB-007351 / NIBIB NIH HHS
Identifiers: 99900546714001842
Language: English
Resource Type: Journal article