Porcine skeletal muscle differentially expressed gene CMYA1: isolation, characterization, mapping, expression and association analysis with carcass traits

X L Xu; X W Xu; P W Pan; K Li; Z H Jiang; M Yu; M F Rothschild; B Liu

doi:10.1111/j.1365-2052.2008.01825.x

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Journal article

Porcine skeletal muscle differentially expressed gene CMYA1: isolation, characterization, mapping, expression and association analysis with carcass traits

X L Xu, X W Xu, P W Pan, K Li, Z H Jiang, M Yu, M F Rothschild and B Liu

Animal genetics, Vol.40(3), pp.255-261

06/2009

DOI: https://doi.org/10.1111/j.1365-2052.2008.01825.x

Handle:

https://hdl.handle.net/2376/112904

PMID: 19170674

Abstract

Swine - metabolism

Gene Frequency

Humans

RNA, Messenger - genetics

Radiation Hybrid Mapping

Reverse Transcriptase Polymerase Chain Reaction - veterinary

Molecular Sequence Data

Gene Expression Profiling

Muscle, Skeletal - physiology

Swine - genetics

DNA-Binding Proteins - genetics

Genetic Variation

RNA, Messenger - biosynthesis

Sequence Alignment

Chromosome Mapping - veterinary

Base Sequence

Cloning, Molecular

Polymorphism, Single Nucleotide

Quantitative Trait Loci

DNA-Binding Proteins - biosynthesis

To investigate the differences in gene expression between some obese and lean pig breeds, differential display of mRNA was employed in our previous research. One differentially expressed EST (BI596262) was further identified as the porcine cardiomyopathy associated 1 (CMYA1) gene because of its homology to the human CMYA1 gene. The full-length DNA of the porcine CMYA1 gene encompasses 9379 bp, including a complete open reading frame encoding 1839 amino acid residues, a 158-bp 5'-untranslated region and a 630-bp 3'-untranslated region. The porcine CMYA1 gene was assigned to chromosome 13 by the radiation hybrid panel (IMpRH). The porcine CMYA1 gene was expressed only in the striated muscle. Single nucleotide polymorphism (SNP) scanning in the coding region identified one synonymous mutation (c.1053C>T) and three missense mutations, c.1394A>G (p.His465Arg), c.1751A>G (p.Asp582Gly) and c.3290C>A (p.Thr1097Asp). The allele frequencies were tested among about 200 unrelated pigs from several pig breeds. Linkage mapping was further conducted with the SNP c.1751A>G (p.Asp582Gly) in a Berkshire x Yorkshire resource family and this confirmed that porcine CMYA1 is closely linked with Sw344 (distance = 2 cM, LOD score is 129.47), an interesting region harbouring a QTL for back fat thickness. Association analysis in our experimental pig population showed that different genotypes of CMYA1 gene were associated with different back fat thicknesses (P < 0.05). Our results suggest that the porcine CMYA1 gene has effects on porcine back fat deposition and further investigation will be necessary to illustrate the underlying mechanisms.

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Title: Porcine skeletal muscle differentially expressed gene CMYA1: isolation, characterization, mapping, expression and association analysis with carcass traits
Creators: X L Xu - Huazhong Agricultural University
X W Xu
P W Pan
K Li
Z H Jiang
M Yu
M F Rothschild
B Liu
Publication Details: Animal genetics, Vol.40(3), pp.255-261
Academic Unit: Department of Biomedical Sciences; Department of Animal Sciences
Publisher: England
Identifiers: 99900547690101842
Language: English
Resource Type: Journal article

Porcine skeletal muscle differentially expressed gene CMYA1: isolation, characterization, mapping, expression and association analysis with carcass traits

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Abstract

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