Journal article
Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells
Planta, Vol.201(3), pp.349-358
03/1997
Handle:
https://hdl.handle.net/2376/109895
PMID: 9129339
Abstract
Distribution of post-translationally modified tubulins in cells of Nicotiana tabacum L. was analysed using a panel of specific antibodies. Polyglutamylated, tyrosinated, nontyrosinated, acetylated and Δ2-tubulin variants were detected on α-tubulin subunits; polyglutamylation was also found on β-tubulin subunits. Modified tubulins were detected by immunofluorescence microscopy in interphase microtubules, preprophase bands, mitotic spindles as well as in phragmoplasts. They were, however, located differently in the various microtubule structures. The antibodies against tyrosinated, acetylated and polyglutamylated tubulins gave uniform staining along all microtubules, while antibodies against nontyrosinated and Δ2-tubulin provided dotlike staining of interphase microtubules. Additionally, immunoreactivity of antibodies against acetylated and Δ2-tubulins was strong in the pole regions of mitotic spindles. High-resolution isoelectric focusing revealed 22 tubulin charge variants in N. tabacum suspension cells. Immunoblotting with antibodies TU-01 and TU-06 against conserved antigenic determinants of α- and β-tubulin molecules, respectively, revealed that 11 isoforms belonged to the α-subunit and 11 isoforms to the β-subunit. Whereas antibodies against polyglutamylated, tyrosinated and acetylated tubulins reacted with several α-tubulin isoforms, antibodies against nontyrosinated and Δ2-tubulin reacted with only one. The combined data demonstrate that plant tubulin is extensively posttranslationally modified and that these modifications participate in the generation of plant tubulin polymorphism.
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Details
- Title
- Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells
- Creators
- Andrey Smertenko - Institute of Cell Biology and Genetic Engineering National Academy of Sciences of the Ukraine Zabolotnogo str. 148, GSP-22 252022 Kiev UkraineYaroslav Blume - Institute of Cell Biology and Genetic Engineering National Academy of Sciences of the Ukraine Zabolotnogo str. 148, GSP-22 252022 Kiev UkraineVladimír Viklický - Institute of Molecular Genetics Academy of Sciences of the Czech Republic Vídeňská 1083 142 20 Praha 4 Czech RepublicZdeněk Opatrný - Institute of Crop Production Drnovská 507 161 06 Praha 6 Czech RepublicPavel Dráber - Institute of Molecular Genetics Academy of Sciences of the Czech Republic Vídeňská 1083 142 20 Praha 4 Czech Republic
- Publication Details
- Planta, Vol.201(3), pp.349-358
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900547299201842
- Language
- English
- Resource Type
- Journal article