Journal article
PrPSc Is Not Detected in Peripheral Blood Leukocytes of Scrapie-Infected Sheep: Determining the Limit of Sensitivity by Immunohistochemistry
Clinical and diagnostic laboratory immunology, Vol.9(2), pp.499-502
03/2002
Handle:
https://hdl.handle.net/2376/115080
PMCID: PMC119962
PMID: 11874903
Abstract
Peripheral blood leukocytes (PBLs) from scrapie-infected sheep were evaluated for the presence of PrP
Sc
by using dissociated retropharyngeal lymph node (DRLN) cells and immunohistochemistry (IHC). PrP
Sc
-positive cells were detected in 2.05% ± 0.28% of 3 × 10
6
DRLN cells, but were not detected in 3 × 10
6
PBLs from scrapie-infected sheep. Titration of DRLN cells mixed with PBLs showed that IHC detects a minimum of 0.00205% or 60 PrP
Sc
-positive cells in 3 × 10
6
PBLs.
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Details
- Title
- PrPSc Is Not Detected in Peripheral Blood Leukocytes of Scrapie-Infected Sheep: Determining the Limit of Sensitivity by Immunohistochemistry
- Creators
- Lynn M Herrmann - USDA Agricultural Research Service ADRUTimothy V Baszler - USDA Agricultural Research Service ADRUDonald P Knowles - USDA Agricultural Research Service ADRUWilliam P Cheevers - USDA Agricultural Research Service ADRU
- Publication Details
- Clinical and diagnostic laboratory immunology, Vol.9(2), pp.499-502
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547928801842
- Language
- English
- Resource Type
- Journal article