Journal article
Pregnancy status and fetal prion genetics determine PrPSc accumulation in placentomes of scrapie-infected sheep
Proceedings of the National Academy of Sciences - PNAS, Vol.99(9), pp.6310-6315
04/30/2002
Handle:
https://hdl.handle.net/2376/115515
PMCID: PMC122945
PMID: 11959902
Abstract
Ovine scrapie is a fatal neurodegenerative disorder that may be transmitted through exposure to infected uterine and placental tissues. Susceptibility to scrapie is primarily controlled by polymorphisms in the prion protein (PrP) gene. Scrapie in the U.S. Suffolk breed and in many breeds in Europe occurs in sheep homozygous for glutamine (171QQ), but rarely in sheep heterozygous for glutamine and arginine (171QR) or homozygous for arginine (171RR) at codon 171 of the PrP gene. This study demonstrated that accumulation of PrP
Sc
in uterine-placental epithelial cells in the placentome was determined by fetal PrP genotype and the pregnancy status of scrapie-infected ewes. PrP
Sc
was detected in 171QQ placentomes of infected ewes, but not in placentomes of infected ewes pregnant with 171QR conceptuses or in the non-pregnant uterus of infected ewes. The distribution of PrP
Sc
plaques in placentomes was temporally associated with stage of gestation. There was a tendency toward increased size and number of placentomal PrP
Sc
plaques from the endometrial stalk (maternal side) to chorionic plate (fetal side). These results indicate that accumulation of PrP
Sc
is eliminated or reduced to undetectable levels in reproductive and placental tissues if infected ewes are not pregnant or conceive conceptuses with a resistant PrP genotype.
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Details
- Title
- Pregnancy status and fetal prion genetics determine PrPSc accumulation in placentomes of scrapie-infected sheep
- Creators
- Wenbin Tuo - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, WA 99164Katherine I O'Rourke - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, WA 99164Dongyue Zhuang - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, WA 99164William P Cheevers - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, WA 99164Terry R Spraker - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, WA 99164Donald P Knowles - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, WA 99164
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.99(9), pp.6310-6315
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- The National Academy of Sciences
- Identifiers
- 99900548181401842
- Language
- English
- Resource Type
- Journal article