Journal article
Prevalence and Antimicrobial Resistance of Thermophilic Campylobacter spp. from Cattle Farms in Washington State
Applied and environmental microbiology, Vol.71(1), pp.169-174
01/2005
Handle:
https://hdl.handle.net/2376/110699
PMCID: PMC544228
PMID: 15640184
Abstract
The prevalence of thermophilic
Campylobacter
spp. was investigated in cattle on Washington State farms. A total of 350 thermophilic
Campylobacter
isolates were isolated from 686 cattle sampled on 15 farms (eight dairies, two calf rearer farms, two feedlots, and three beef cow-calf ranches). Isolate species were identified with a combination of phenotypic tests,
hipO
colony blot hybridization, and multiplex
lpxA
PCR. Breakpoint resistance to four antimicrobials (ciprofloxacin, nalidixic acid, erythromycin, and doxycycline) was determined by agar dilution.
Campylobacter jejuni
was the most frequent species isolated (34.1%), followed by
Campylobacter coli
(7.7%) and other thermophilic campylobacters (1.5%). The most frequently detected resistance was to doxycycline (42.3% of 350 isolates). Isolates from calf rearer facilities were more frequently doxycycline resistant than isolates from other farm types.
C. jejuni
was most frequently susceptible to all four of the antimicrobial drugs studied (58.8% of 272 isolates).
C. coli
isolates were more frequently resistant than
C. jejuni
, including resistance to quinolone antimicrobials (89.3% of isolates obtained from calves on calf rearer farms) and to erythromycin (72.2% of isolates obtained from feedlot cattle). Multiple drug resistance was more frequent in
C. coli
(51.5%) than in
C. jejuni
(5.1%). The results of this study demonstrate that
C. jejuni
is widely distributed among Washington cattle farms, while
C. coli
is more narrowly distributed but significantly more resistant.
Metrics
9 Record Views
Details
- Title
- Prevalence and Antimicrobial Resistance of Thermophilic Campylobacter spp. from Cattle Farms in Washington State
- Creators
- Wonki Bae - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of KoreaKatherine N Kaya - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of KoreaDale D Hancock - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of KoreaDouglas R Call - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of KoreaYong Ho Park - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of KoreaThomas E Besser - Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea
- Publication Details
- Applied and environmental microbiology, Vol.71(1), pp.169-174
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547167201842
- Language
- English
- Resource Type
- Journal article