Journal article
Primary and Secondary Lysosomes in Megakaryocytes and Platelets from Cattle with the Chediak-Higashi Syndrome
Thrombosis and haemostasis, Vol.64(1), pp.156-160
1990
Handle:
https://hdl.handle.net/2376/113952
PMID: 2274921
Abstract
Summary
The ultrastructure of lysosomes from megakaryocytes (MK) and platelets of cattle with the Chediak-Higashi syndrome (CHS) was characterized using acid phosphatase histochemistry with beta-glycerophosphate as substrate and cerium as a capturing agent. Acid phosphatase was localized in the trans aspect of the Golgi complex and/or granules in MK at all stages of maturation. Morphometric analysis of the diameter of each lysosome was performed on MK from CHS cattle and compared to MK from normal cattle. Lysosomes in CHS MK were neither enlarged nor different with respect to classification as secondary lysosomes, which composed 35% of the lysosomes in CHS MK. Lysosomes were demonstrated in 22% of the CHS platelet sections and appeared similar to those from normal cattle, 56% of them being classified as secondary lysosomes. Why lysosomes are not enlarged in bovine CHS MK and platelets, whereas they are enlarged in most other cell types, remains unknown.
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Details
- Title
- Primary and Secondary Lysosomes in Megakaryocytes and Platelets from Cattle with the Chediak-Higashi Syndrome
- Creators
- Michèle Ménard - Department of Veterinary Clinical Medicine and SurgeryKenneth M Meyers - The Departments of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, WA, U.SDavid J Prieur - Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, U.S.A
- Publication Details
- Thrombosis and haemostasis, Vol.64(1), pp.156-160
- Publisher
- Schattauer GmbH; Stuttgart
- Identifiers
- 99900548484001842
- Language
- English
- Resource Type
- Journal article