Journal article
Prime-boost vaccination with plasmid DNA encoding caprine-arthritis encephalitis lentivirus env and viral SU suppresses challenge virus and development of arthritis
Virology (New York, N.Y.), Vol.306(1), pp.116-125
2003
Handle:
https://hdl.handle.net/2376/102108
PMID: 12620804
Abstract
This study evaluated the efficacy of prime-boost vaccination for immune control of caprine arthritis-encephalitis virus (CAEV), a macrophage tropic lentivirus that causes progressive arthritis in the natural host. Vaccination of Saanen goats with pUC-based plasmid DNA expressing CAEV
env induces T helper type 1 (Th1) biased immune responses to vector-encoded surface envelope (SU), and the plasmid-primed Th1 response is expanded following boost with purified SU in Freund’s incomplete adjuvant (SU-FIA) (J. C. Beyer et al., 2001, Vaccine 19, 1643-1651). Four goats vaccinated with
env expression plasmids and boosted with SU-FIA were challenged intravenously with 1 × 10
4 TCID
50 of CAEV at 428 days after SU-FIA boost and evaluated by immunological, virological, and disease criteria. Controls included two goats primed with pUC18 and eight unvaccinated goats. Goats receiving prime-boost vaccination with CAEV
env plasmids and SU-FIA became infected but suppressed postchallenge virus replication, provirus loads in lymph node, and development of arthritis for at least 84 weeks.
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Details
- Title
- Prime-boost vaccination with plasmid DNA encoding caprine-arthritis encephalitis lentivirus env and viral SU suppresses challenge virus and development of arthritis
- Creators
- W.P Cheevers - Department of Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USAK.R Snekvik - Department of Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USAJ.D Trujillo - Department of Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USAN.M Kumpula-McWhirter - Department of Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USAK.J Pretty On Top - Department of Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USAD.P Knowles - Department of Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Virology (New York, N.Y.), Vol.306(1), pp.116-125
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900546634901842
- Language
- English
- Resource Type
- Journal article