Journal article
Probing RegA/RNA Interactions Using Electrospray Ionization–Fourier Transform Ion Cyclotron Resonance–Mass Spectrometry
Analytical biochemistry, Vol.262(1), pp.67-76
08/15/1998
Handle:
https://hdl.handle.net/2376/113957
PMID: 9735149
Abstract
The interactions of bacteriophage T4 regA protein, a unique translational regulator, with RNAs of various size and sequence were studied using electrospray ionization–Fourier transform ion cyclotron resonance–mass spectrometry. Using very gentle interface conditions, regA/RNA complexes with a 1:1 binding stoichiometry were observed for all four target RNAs studied, consistent with solution binding studies. Competitive binding of target RNAs and their degradation products with regA demonstrated that the loss of a single nucleotide resulted in a dramatic change in binding affinity in some cases. Competitive binding of regA with four target RNAs revealed similar relative binding affinity order to that suggested by previousin vitrorepression experiments. The use of sustained off-resonance irradiation for collisionally induced dissociation of a regA/RNA complex suggested the potential for directly obtaining information regarding the regA binding domain.
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Details
- Title
- Probing RegA/RNA Interactions Using Electrospray Ionization–Fourier Transform Ion Cyclotron Resonance–Mass Spectrometry
- Creators
- Chuanliang Liu - Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, 99352Ljiljana Paša Tolić - Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, 99352Steven A Hofstadler - Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, 99352Amy C Harms - Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, 99352Richard D Smith - Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, 99352ChulHee Kang - Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington, 99164Nanda Sinha - Boston Biosystems, Inc. Bedford, Massachusetts, 01730
- Publication Details
- Analytical biochemistry, Vol.262(1), pp.67-76
- Academic Unit
- Chemistry, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547411601842
- Language
- English
- Resource Type
- Journal article