Journal article
Production of Organic Acids by Probiotic Lactobacilli Can Be Used to Reduce Pathogen Load in Poultry
PloS one, Vol.7(9), pp.e43928-e43928
09/04/2012
Handle:
https://hdl.handle.net/2376/100287
PMCID: PMC3433458
PMID: 22962594
Abstract
Probiotic
Lactobacillus
can be used to reduce the colonization of pathogenic bacteria in food animals, and therefore reduce the risk of foodborne illness to consumers. As a model system, we examined the mechanism of protection conferred by
Lactobacillus
species to inhibit
C. jejuni
growth
in vitro
and reduce colonization in broiler chickens. Possible mechanisms for the reduction of pathogens by lactobacilli include: 1) stimulation of adaptive immunity; 2) alteration of the cecal microbiome; and, 3) production of inhibitory metabolites, such as organic acids. The
Lactobacillus
species produced lactic acid at concentrations sufficient to kill
C. jejuni in vitro
. We determined that lactic acid produced by
Lactobacillus
disrupted the membrane of
C. jejuni
, as judged by biophotonics. The spectral features obtained using Fourier-transform infrared (FT-IR) and Raman spectroscopy techniques were used to accurately predict bacterial viability and differentiate
C. jejuni
samples according to lactic acid treatment. FT-IR spectral features of
C. jejuni
and
Lactobacillus
grown in co-culture revealed that the metabolism was dominated by
Lactobacillus
prior to the killing of
C. jejuni
. Based on our results, the development of future competitive exclusion strategies should include the evaluation of organic acid production.
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Details
- Title
- Production of Organic Acids by Probiotic Lactobacilli Can Be Used to Reduce Pathogen Load in Poultry
- Creators
- Jason M Neal-McKinneyXiaonan LuTri DuongCharles L LarsonDouglas R CallDevendra H ShahMichael E Konkel
- Publication Details
- PloS one, Vol.7(9), pp.e43928-e43928
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health; Molecular Biosciences, School of
- Publisher
- Public Library of Science; San Francisco, USA
- Identifiers
- 99900546571201842
- Language
- English
- Resource Type
- Journal article