Journal article
Production of anti-fungal volatiles by non-pathogenic Fusarium oxysporum and its efficacy in suppression of Verticillium wilt of cotton
Plant and soil, Vol.392(1), pp.101-114
07/2015
Handle:
https://hdl.handle.net/2376/115658
Abstract
The study aimed at identifying volatile organic compounds (VOCs) produced by the non-pathogenic Fusarium oxysporum (Fo) strain CanR-46, and to determine the anti-fungal spectrum and the control efficacy of the Fo-VOCs.The Fo-VOCs were identified by GC-MS. The antifungal activity of the Fo-VOCs was tested using the double-dish method. Soil contaminated with Verticillium dahliae was fumigated with the Fo-VOCs to test the control efficacy. The GFP-tagged derivative strain CanR-46GFP was tested to colonize cotton roots for prevention of infection by V. dahliae.
Nineteen VOCs were identified with eremophila-1 (10),11-diene being the most abundant. The Fo-VOCs inhibited mycelial growth of 14 fungal species including V. dahliae, delayed V. dahliae conidial germination, suppressed V. dahliae germ-tube elongation, and caused V. dahliae hyphal shriveling and collapse. Four synthetic chemicals, 5-hexenoic acid, limonene, octanoic acid and 3,4-2H-dihydropyran, in the Fo-VOCs profile showed antifungal activity against V. dahliae. The Fo-VOCs significantly (P < 0.05) reduced severity of Verticillium wilt of cotton compared to the control. CanR-46GFP extensively colonized the cotton root tissues, thus effectively prevented V. dahliae infection.The VOC-producing fungus F. oxysporum CanR-46 is a promising biocontrol agent against V. dahliae.
Metrics
30 Record Views
Details
- Title
- Production of anti-fungal volatiles by non-pathogenic Fusarium oxysporum and its efficacy in suppression of Verticillium wilt of cotton
- Creators
- Qinghua Zhang - The Institute of Vegetables and Flowers Jiangxi Academy of Agricultural Sciences Nanchang 330200 Jiangxi Province ChinaLong Yang - State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province Huazhong Agricultural University Wuhan 430070 ChinaJing Zhang - State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province Huazhong Agricultural University Wuhan 430070 ChinaMingde Wu - State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province Huazhong Agricultural University Wuhan 430070 ChinaWeidong Chen - United States Department of Agriculture, Agricultural Research Service Washington State University Pullman WA USADaohong Jiang - State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province Huazhong Agricultural University Wuhan 430070 ChinaGuoqing Li - State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant, Pathology of Hubei Province Huazhong Agricultural University Wuhan 430070 China
- Contributors
- Huaiying Yao (Editor)Yongguan Zhu (Editor)
- Publication Details
- Plant and soil, Vol.392(1), pp.101-114
- Academic Unit
- Plant Pathology, Department of
- Publisher
- Springer International Publishing; Cham
- Identifiers
- 99900548396001842
- Language
- English
- Resource Type
- Journal article