Journal article
Production of cellulase by Trichoderma reesei from dairy manure
Bioresource technology, Vol.96(4), pp.491-499
03/2005
Handle:
https://hdl.handle.net/2376/103349
PMID: 15491832
Abstract
Cellulase production by the fungi Trichoderma reesei was studied using dairy manure as a substrate. Data showed that T. reesei RUT-C30 had higher cellulase production than T. reesei QM 9414 and that a homogenized manure, treated by a blender to reduce fiber size, led to higher cellulase production. The cellulase production was further optimized by growing T. reesei RUT-C30 on homogenized manure. The effects of manure concentration, pH, and temperature on cellulase production were investigated with optimal parameter values determined to be 10 g/l manure (dry basis), 25.5 degrees C, and pH 5.7, respectively. Elimination of CaCl2, MgSO4, nitrogen sources (NH4+ and urea) and trace elements (Fe2+, Zn2+, Co2+ and Mn2+) from the original salt solution had no negative influence on the cellulase production, while phosphate elimination did reduce cellulase production. Based on above results, the final medium composition was simplified with manure additives being KH2PO4, tween-80 and CoCl2 only. Using this medium composition and a reaction time of 6-8 days, a maximum cellulase production activity of 1.74 IU/ml of filter paper activity, 12.22 IU/ml of CMCase activity, and 0.0978 IU/ml of beta-glucosidase was obtained. This filter paper activity is the highest ever reported in cellulase production from agricultural wastes.
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Details
- Title
- Production of cellulase by Trichoderma reesei from dairy manure
- Creators
- Zhiyou Wen - Department of Biological Systems Engineering, Center of Multiphase Environmental Research, Washington State University, Pullman, WA 99164, USA. zwen@wsu.eduWei LiaoShulin Chen
- Publication Details
- Bioresource technology, Vol.96(4), pp.491-499
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- England
- Identifiers
- 99900546680301842
- Language
- English
- Resource Type
- Journal article