Journal article
Production of huperzine A and other Lycopodium alkaloids in Huperzia species grown under controlled conditions and in vitro
Phytochemistry (Oxford), Vol.91, pp.208-219
07/2013
Handle:
https://hdl.handle.net/2376/107089
PMID: 23306162
Abstract
LC–IMS–MS analysis of Huperzia species grown under controlled conditions sheds light on the regulation of Lycopodium alkaloid biosynthesis. H. pinifolia callus produces nankakurine B instead of huperzine A. [Display omitted]
► Huperzia species vary greatly in Lycopodium alkaloid composition and content. ► Controlled growth conditions are important for evaluating alkaloid variation. ► Genetics and the environment play important roles in controlling HupA production. ► H. pinifolia switched from HupA to nankakurine production when callus was induced. ► UPLC–IMS-TOFMS is ideally suited for rapid quantification of Lycopodium alkaloids.
A UPLC–MS method was developed for quantifying huperzine A (HupA), an anti-Alzheimer’s disease (AD) drug candidate from the traditional Chinese medicine Qian Ceng Ta (Huperzia serrata), in samples of 11 Huperzia genus plants. The highest content of HupA was found in Huperzia pinifolia. The accumulation of various Lycopodium alkaloids was monitored in these tissues using high resolution Q-IMS-TOFMS analysis. Tissue culture of various Huperzia species has been achieved and production of HupA has been confirmed in the callus of H. pinifolia. Furthermore, it was established that the major alkaloid produced by the naturally grown plant and the callus of H. pinifolia changed dramatically from HupA to nankakurine B.
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Details
- Title
- Production of huperzine A and other Lycopodium alkaloids in Huperzia species grown under controlled conditions and in vitro
- Creators
- Kan’ichiro IshiuchiJeong-Jin ParkRobert M LongDavid R Gang
- Publication Details
- Phytochemistry (Oxford), Vol.91, pp.208-219
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900546959601842
- Language
- English
- Resource Type
- Journal article