Profiling gene expression during the differentiation and development of the murine embryonic gonad

Christopher L Small; James E Shima; Mehmet Uzumcu; Michael K Skinner; Michael D Griswold

doi:10.1095/biolreprod.104.033696

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Profiling gene expression during the differentiation and development of the murine embryonic gonad

Journal article

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Profiling gene expression during the differentiation and development of the murine embryonic gonad

Christopher L Small, James E Shima, Mehmet Uzumcu, Michael K Skinner and Michael D Griswold

Biology of reproduction, Vol.72(2), pp.492-501

02/2005

DOI: https://doi.org/10.1095/biolreprod.104.033696

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https://hdl.handle.net/2376/116249

PMCID: PMC3217241

PMID: 15496517

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Abstract

Gonads - embryology

Testis - metabolism

Oligonucleotide Array Sequence Analysis

Ovary - embryology

Gene Expression Profiling

Transcription Factors - genetics

Reverse Transcriptase Polymerase Chain Reaction

RNA - genetics

RNA - biosynthesis

Gonads - metabolism

Testis - embryology

Genes, sry - genetics

SOX9 Transcription Factor

Cluster Analysis

High Mobility Group Proteins - genetics

Ovary - metabolism

Pregnancy

Phenotype

The application of microarray technology to the study of mammalian organogenesis can provide greater insights into the steps necessary to elicit a functionally competent tissue. To this end, a temporal profile of gene expression was generated with the purpose of identifying changes in gene expression occurring within the developing male and female embryonic gonad. Gonad tissue was collected from mouse embryos at 11.5, 12.5, 14.5, 16.5, and 18.5 days postcoitum (dpc) and relative steady-state levels of mRNA were determined using the Affymetrix MGU74v2 microarray platform. Statistical analysis produced 3693 transcripts exhibiting differential expression during male and/or female gonad development. At 11.5 dpc, the gonad is morphologically indifferent, but at 12.5 dpc, transitions to a male or female phenotype are discernible by the appearance of testicular cords. A number of genes are expressed during this period and many share similar expression profiles in both sexes. As expected, the expression of two well-known sex determination genes, specifically Sry and Sox9, is unique to the testis. Beyond 12.5 dpc, differential gene expression becomes increasingly evident as the male and female tissue morphologically and physiologically diverges. This is evident by two unique waves of transcriptional activity occurring after 14.5 dpc in the male and female. With this study, a large number of transcripts comprising the murine transcriptome can be examined throughout male and female embryonic gonad development and allow for a more complete description of gonad differentiation and development.

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Title: Profiling gene expression during the differentiation and development of the murine embryonic gonad
Creators: Christopher L Small - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164, USA
James E Shima
Mehmet Uzumcu
Michael K Skinner
Michael D Griswold
Publication Details: Biology of reproduction, Vol.72(2), pp.492-501
Academic Unit: Biological Sciences, School of; Molecular Biosciences, School of
Publisher: United States
Grant note: U54 HD042454 / NICHD NIH HHS R37 HD010808 / NICHD NIH HHS R01 HD010808 / NICHD NIH HHS U54 HD 42454 / NICHD NIH HHS R01 HD010808-32 / NICHD NIH HHS HD 10808 / NICHD NIH HHS
Identifiers: 99900547980701842
Language: English
Resource Type: Journal article