Prolonged androgen deprivation leads to overexpression of calpain 2: implications for prostate cancer progression

Tiancheng Liu; Desiree E Mendes; Clifford E Berkman

doi:10.3892/ijo.2013.2196

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Prolonged androgen deprivation leads to overexpression of calpain 2: implications for prostate cancer progression

Journal article

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Peer reviewed

Prolonged androgen deprivation leads to overexpression of calpain 2: implications for prostate cancer progression

Tiancheng Liu, Desiree E Mendes and Clifford E Berkman

International journal of oncology, Vol.44(2), pp.467-472

02/2014

DOI: https://doi.org/10.3892/ijo.2013.2196

Handle:

https://hdl.handle.net/2376/114827

PMCID: PMC3898865

PMID: 24297527

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Abstract

Prostatic Neoplasms - metabolism

Prostatic Neoplasms - pathology

Calpain - metabolism

Humans

Receptors, Androgen - metabolism

Male

Blotting, Western

Immunoenzyme Techniques

Microscopy, Confocal

Filamins - metabolism

Fluorescent Antibody Technique

Dipeptides - metabolism

Tumor Cells, Cultured

Androgens - metabolism

Calpain - antagonists & inhibitors

Understanding the molecular mechanism of prostate cancer progression from androgen dependence to independence may lead to developing more effective treatments against prostate cancer. Herein, our previous in vitro model was employed to assess the effects of continuous androgen-deprivation on developing the metastatic phenotype from androgen-dependent prostate cancer cells (LNCaP). The results indicated that long-term androgen deprivation resulted in overexpression of calpain 2 and increased expression of filamin A (FlnA), but not for calpain 1. The enhanced activity of calpain 2 was confirmed by the accumulation of cleaved FlnA fragments, which could be effectively blocked by calpeptin (an inhibitor of calpain 2). Therefore, the combination of calpain 2 inhibitor and androgen deprivation may provide new therapeutic strategy for patients to prevent or postpone prostate cancer progression.

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Title: Prolonged androgen deprivation leads to overexpression of calpain 2: implications for prostate cancer progression
Creators: Tiancheng Liu - Department of Chemistry, Washington State University, Pullman, WA 99164, USA
Desiree E Mendes - Department of Chemistry, Washington State University, Pullman, WA 99164, USA
Clifford E Berkman - Department of Chemistry, Washington State University, Pullman, WA 99164, USA
Publication Details: International journal of oncology, Vol.44(2), pp.467-472
Academic Unit: Chemistry, Department of
Publisher: Greece
Grant note: R01CA140617 / NCI NIH HHS R01 CA140617 / NCI NIH HHS T32 GM008336 / NIGMS NIH HHS
Identifiers: 99900547776201842
Language: English
Resource Type: Journal article