Journal article
Prominence of an O75 Clonal Group (Clonal Complex 14) among Non-ST131 Fluoroquinolone-Resistant Escherichia coli Causing Extraintestinal Infections in Humans and Dogs in Australia
Antimicrobial agents and chemotherapy, Vol.56(7), pp.3898-3904
07/2012
Handle:
https://hdl.handle.net/2376/103615
PMCID: PMC3393427
PMID: 22526317
Abstract
Fluoroquinolone (FQ)-resistant extraintestinal pathogenic
Escherichia coli
(FQ
r
ExPEC) strains from phylogenetic group B2 are undergoing epidemic spread. Isolates belonging to phylogenetic group B2 are generally more virulent than other
E. coli
isolates; therefore, resistance to FQs among group B2 isolates is concerning. Although clonal expansion of sequence type 131 (ST131) is a major factor, the contribution of additional clonal groups has not been quantified. Group B2 FQ
r
ExPEC isolates from humans (
n
= 250) and dogs (
n
= 12) in Australia were screened for ST131, a recently recognized and rapidly emerging multidrug-resistant and virulent clonal group that is important in both human and companion animal medicine. Non-ST131 isolates underwent virulence genotyping, PCR-based O typing, partial multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and FQ resistance mechanism analysis. Of 49 non-ST131 isolates (45 human, 4 canine), 49% (24 human, 2 canine) represented O-type O75 and exhibited conserved virulence genotypes (F10
papA
allele,
iha
,
fimH
,
sat
,
vat
,
fyuA
,
iutA
,
kpsM
II,
usp
,
ompT
,
malX
, K1/K5 capsule) and MLST allele profiles corresponding with clonal complex CC14. Two clusters, each containing canine and human isolates, were identified by PFGE (differentiated by K1 and K5 capsules). Australian FQ
r
O75 isolates exhibited commonality with an historical FQ-susceptible O75 urosepsis isolate (also CC14). The isolation from humans and dogs of highly similar FQ
r
derivatives of the classic O75:K1/K5 (CC14) ExPEC lineage suggests recent acquisition of FQ resistance and potential cross-host-species transfer. This lineage should be targeted with ST131 in future epidemiological investigations of FQ
r
ExPEC.
Metrics
5 Record Views
Details
- Title
- Prominence of an O75 Clonal Group (Clonal Complex 14) among Non-ST131 Fluoroquinolone-Resistant Escherichia coli Causing Extraintestinal Infections in Humans and Dogs in Australia
- Creators
- Joanne L Platell - School of Veterinary Science, University of Queensland, Gatton, Queensland, AustraliaDarren J Trott - School of Veterinary Science, University of Queensland, Gatton, Queensland, AustraliaJames R Johnson - Veterans Affairs Medical Center and University of Minnesota, Minneapolis, Minnesota, USAPeter Heisig - Institute of Biochemistry and Molecular Biology, Pharmaceutical Biology and Microbiology, University of Hamburg, Hamburg, GermanyAnke Heisig - Institute of Biochemistry and Molecular Biology, Pharmaceutical Biology and Microbiology, University of Hamburg, Hamburg, GermanyConnie R Clabots - Veterans Affairs Medical Center and University of Minnesota, Minneapolis, Minnesota, USABrian Johnston - Veterans Affairs Medical Center and University of Minnesota, Minneapolis, Minnesota, USARowland N Cobbold - School of Veterinary Science, University of Queensland, Gatton, Queensland, Australia
- Publication Details
- Antimicrobial agents and chemotherapy, Vol.56(7), pp.3898-3904
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Identifiers
- 99900546799601842
- Language
- English
- Resource Type
- Journal article