Journal article
Prospects for functional analysis of effectors from cereal rust fungi
Euphytica, Vol.179(1), pp.57-67
05/2011
Handle:
https://hdl.handle.net/2376/109119
Abstract
With the advancement of several Puccinia genome sequencing projects, along with gene expression data and methods for predicting secreted proteins, it is now possible to predict many effector proteins from the cereal rusts. Biological assays that can be conducted in a relatively high throughput fashion are necessary to assign specific functions, such as avirulence. Biolistic delivery of potential effectors is limited by the need to examine individual cells and delivery by Agrobacterium generally also affects small numbers of cells in grasses. An approach that has had some success in dicots is the use of bacterial systems to deliver proteins by their type III secretion systems (TTSS). Several bacterial systems were thus tested for their suitability in delivering effectors to wheat. Pseudomonas syringae DC3000 caused hypersensitive reactions (HR) when infiltrated into all tested wheat lines but only some barley lines. A variant strain with multiple effectors deleted showed a reduced HR on wheat lines. Pseudomonas fluorescens with an engineered TTSS system showed no HR in wheat lines but was able to deliver bacterial effectors AvrRpm1 and AvrRpt2 and the fungal toxin ToxA. Delivery of the effectors by P. fluorescens could be detected by HR or by staining for presence of hydrogen peroxide or callose deposits. The bacterial systems thus showed good potential for their ability to deliver foreign proteins into wheat cells.
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Details
- Title
- Prospects for functional analysis of effectors from cereal rust fungi
- Creators
- Chuntao Yin - Department of Plant Pathology Washington State University Pullman WA 99164-6430 USAScot Hulbert - Department of Plant Pathology Washington State University Pullman WA 99164-6430 USA
- Publication Details
- Euphytica, Vol.179(1), pp.57-67
- Academic Unit
- Plant Pathology, Department of
- Publisher
- Springer Netherlands; Dordrecht
- Identifiers
- 99900547490101842
- Language
- English
- Resource Type
- Journal article