Journal article
Protective immunity induced by immunization with a live, cultured Anaplasma marginale strain
Vaccine, Vol.31(35), pp.3617-3622
08/02/2013
Handle:
https://hdl.handle.net/2376/107323
PMCID: PMC3903126
PMID: 23664994
Abstract
•AmStM-GFP is a marked, culture derived, live vaccine candidate for anaplasmosis.•AmStM-GFP and A. centrale result in similar levels of bacteremia and anemia.•AmStM-GFP provides protection against disease following homologous challenge.
Despite significant economic losses resulting from infection with Anaplasma marginale, a tick-transmitted rickettsial pathogen of cattle, available vaccines provide, at best, only partial protection against clinical disease. The green-fluorescent protein expressing mutant of the A. marginale St. Maries strain is a live, marked vaccine candidate (AmStM-GFP). To test whether AmStM-GFP is safe and provides clinical protection, a group of calves was vaccinated, and clinical parameters, including percent parasitized erythrocytes (PPE), packed cell volume (PCV) and days required to reach peak bacteremia, were measured following inoculation and following tick challenge with wild type St. Maries strain (AmStM). These clinical parameters were compared to those obtained during infection with the A. marginale subsp. centrale vaccine strain (A. centrale) or wild type AmStM. AmStM-GFP resulted in similar clinical parameters to A. centrale, but had a lower maximum PPE, smaller drop in PCV and took longer to reach peak bacteremia than wild type AmStM. AmStM-GFP provided clinical protection, yielding a stable PCV and low bacteremia following challenge, whereas A. centrale only afforded partial clinical protection.
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Details
- Title
- Protective immunity induced by immunization with a live, cultured Anaplasma marginale strain
- Creators
- G. Kenitra Hammac - Program in Genomics, Department of Veterinary Microbiology and Pathology, Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164-7040, USAPei-Shin Ku - Program in Genomics, Department of Veterinary Microbiology and Pathology, Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164-7040, USAMaria F Galletti - Program in Genomics, Department of Veterinary Microbiology and Pathology, Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164-7040, USASusan M Noh - Animal Disease Research Unit, U.S. Department of Agriculture, Agricultural Research Service, PO Box 646630, Pullman, WA 99164-6630, USAGlen A Scoles - Animal Disease Research Unit, U.S. Department of Agriculture, Agricultural Research Service, PO Box 646630, Pullman, WA 99164-6630, USAGuy H Palmer - Program in Genomics, Department of Veterinary Microbiology and Pathology, Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164-7040, USAKelly A Brayton - Program in Genomics, Department of Veterinary Microbiology and Pathology, Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA 99164-7040, USA
- Publication Details
- Vaccine, Vol.31(35), pp.3617-3622
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier Ltd
- Identifiers
- 99900546752901842
- Language
- English
- Resource Type
- Journal article