Journal article
Protein Kinase A Signaling Inhibits Iridophore Differentiation in Zebrafish
Journal of developmental biology, Vol.6(4), p.23
12/01/2018
Handle:
https://hdl.handle.net/2376/106685
PMCID: PMC6315511
PMID: 30261583
Abstract
In zebrafish (
Danio rerio
), iridophores are specified from neural crest cells and represent a tractable system for examining mechanisms of cell fate and differentiation. Using this system, we have investigated the role of cAMP protein kinase A (PKA) signaling in pigment cell differentiation. Activation of PKA with the adenylyl cyclase activator forskolin reduces the number of differentiated iridophores in wildtype larvae, with insignificant changes to melanophore number. Inhibition of PKA with H89 significantly increases iridophore number, supporting a specific role for PKA during iridophore development. To determine the effects of altering PKA activity on iridophore and melanophore gene expression, we examined expression of iridophore marker
pnp4a
, melanophore marker
mitfa
, and the
mitfa
repressor
foxd3
. Consistent with our cell counts, forskolin significantly decreased
pnp4a
expression as detected by in situ hybridization and quantification of
pnp4a+
cells. Forskolin had the opposite effect on
mitfa
and
foxd3
gene activity, increasing the area of expression. As
mitfa/nacre
mutants have extra iridophores as compared to wildtype larvae, we examined the function of
mitfa
during PKA-sensitive iridophore development. Forskolin treatment of
mitfa/nacre
mutants did significantly reduce the number of iridophores but to a lesser extent than that observed in treated wildtype larvae. Taken together, our data suggests that PKA inhibits iridophore development in a subset of iridophore precursors, potentially via a
foxd3
-independent pathway.
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Details
- Title
- Protein Kinase A Signaling Inhibits Iridophore Differentiation in Zebrafish
- Creators
- Cynthia D Cooper - School of Molecular Biosciences, Washington State University Vancouver, Vancouver, WA 98686, USASteve D Erickson - College of Arts and Sciences, Washington State University Vancouver, Vancouver, WA 98686, USAScott Yin - College of Arts and Sciences, Washington State University Vancouver, Vancouver, WA 98686, USATrevor Moravec - College of Arts and Sciences, Washington State University Vancouver, Vancouver, WA 98686, USABrian Peh - College of Arts and Sciences, Washington State University Vancouver, Vancouver, WA 98686, USAKevin Curran - Department of Biology, University of San Diego, San Diego, CA 92110, USA
- Publication Details
- Journal of developmental biology, Vol.6(4), p.23
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- MDPI
- Identifiers
- 99900546843801842
- Language
- English
- Resource Type
- Journal article