Journal article
Protein separation using preparative‐scale dynamic field gradient focusing
Electrophoresis, Vol.29(13), pp.2820-2827
07/13/2008
Handle:
https://hdl.handle.net/2376/106080
PMID: 18615787
Abstract
Dynamic field gradient focusing uses an electric field gradient to separate and concentrate proteins in native buffers. A prototype preparative‐scale dynamic field gradient focusing apparatus reproducibly separated hemoglobin and bovine serum albumin with a mean resolution of 2.64 ± 0.503. Run‐to‐run variations in the hemoglobin's focal point and peak width appeared to be related to fluctuations in the shape of the electric field, rather than the 5% accuracy of the pump that provided the counter‐flow in the separation annulus. The variation in the electric field gradient was probably due to the formation and expansion of an ion‐depleted region at the top of the separation annulus.
Metrics
10 Record Views
Details
- Title
- Protein separation using preparative‐scale dynamic field gradient focusing
- Creators
- Noah I TracyCornelius F Ivory
- Publication Details
- Electrophoresis, Vol.29(13), pp.2820-2827
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- WILEY‐VCH Verlag; Weinheim
- Number of pages
- 8
- Grant note
- Washington State University National Institutes of Health Protein Biotechnology Training Program (T32GM08336) National Science Foundation (BES 9970972) Berlex Laboratories (now a subsidiary of Bayer HealthCare Pharmaceuticals)
- Identifiers
- 99900546808201842
- Language
- English
- Resource Type
- Journal article