Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance

Jayaveeramuthu Nirmala; Stephanie Dahl; Brian J Steffenson; C. Gamini Kannangara; Diter von Wettstein; Xianming Chen; Andris Kleinhofs

doi:10.1073/pnas.0703758104

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Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance

Journal article

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Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance

Jayaveeramuthu Nirmala, Stephanie Dahl, Brian J Steffenson, C. Gamini Kannangara, Diter von Wettstein, Xianming Chen and Andris Kleinhofs

Proceedings of the National Academy of Sciences - PNAS, Vol.104(24), pp.10276-10281

06/12/2007

DOI: https://doi.org/10.1073/pnas.0703758104

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https://hdl.handle.net/2376/109728

PMCID: PMC1891204

PMID: 17548826

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Abstract

Biological Sciences

avirulence

Puccinia graminis

cultivar

programmed cell death

In plants, disease resistance mediated by the gene-for-gene mechanism involves the recognition of specific effector molecules produced by the pathogen either directly or indirectly by the resistance-gene products. This recognition triggers a series of signals, thereby serving as a molecular switch in regulating defense mechanisms by the plants. To understand the mechanism of action of the barley stem rust resistance gene Rpg1 , we investigated the fate of the RPG1 protein in response to infection with the stem rust fungus, Puccinia graminis f. sp. tritici . The investigations revealed that RPG1 disappears to undetectable limits only in the infected tissues in response to avirulent, but not virulent pathotypes. The RPG1 protein disappearance is rapid and appears to be due to specific protein degradation via the proteasome-mediated pathway as indicated by inhibition with the proteasomal inhibitor MG132, but not by other protease inhibitors.

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Title: Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance
Creators: Jayaveeramuthu Nirmala - Departments of Crop and Soil Sciences and
Stephanie Dahl - Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108
Brian J Steffenson - Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108
C. Gamini Kannangara - Departments of Crop and Soil Sciences and
Diter von Wettstein - Departments of Crop and Soil Sciences and
Xianming Chen - Plant Pathology, U.S. Department of Agriculture–Agricultural Research Service and
Andris Kleinhofs - Departments of Crop and Soil Sciences and
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.104(24), pp.10276-10281
Academic Unit: Crop and Soil Sciences, Department of; Plant Pathology, Department of
Publisher: National Academy of Sciences
Identifiers: 99900546910401842
Language: English
Resource Type: Journal article