Journal article
Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida
Applied and environmental microbiology, Vol.78(18), pp.6683-6688
09/2012
Handle:
https://hdl.handle.net/2376/105276
PMCID: PMC3426682
PMID: 22798357
Abstract
Mannheimia haemolytica
,
Pasteurella multocida
, and
Bibersteinia trehalosi
have been identified in the lungs of pneumonic bighorn sheep (BHS;
Ovis canadensis
). Of these pathogens,
M. haemolytica
has been shown to consistently cause fatal pneumonia in BHS under experimental conditions. However,
M. haemolytica
has been isolated by culture less frequently than the other bacteria. We hypothesized that the growth of
M. haemolytica
is inhibited by other bacteria in the lungs of BHS. The objective of this study was to determine whether
P. multocida
inhibits the growth of
M. haemolytica
. Although in monoculture both bacteria exhibited similar growth characteristics, in coculture with
P. multocida
there was a clear inhibition of growth of
M. haemolytica
. The inhibition was detected at mid-log phase and continued through the stationary phase. When cultured in the same medium, the growth of
M. haemolytica
was inhibited when both bacteria were separated by a membrane that allowed contact (pore size, 8.0 μm) but not when they were separated by a membrane that limited contact (pore size, 0.4 μm). Lytic bacteriophages or bactericidal compounds could not be detected in the culture supernatant fluid from monocultures of
P. multocida
or from
P. multocida
-
M. haemolytica
cocultures. These results indicate that
P. multocida
inhibits the growth of
M. haemolytica
by a contact- or proximity-dependent mechanism. If the inhibition of growth of
M. haemolytica
by
P. multocida
occurs
in vivo
as well, it could explain the inconsistent isolation of
M. haemolytica
from the lungs of pneumonic BHS.
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Details
- Title
- Proximity-Dependent Inhibition of Growth of Mannheimia haemolytica by Pasteurella multocida
- Creators
- Jegarubee Bavananthasivam - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USARohana P Dassanayake - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USAAbirami Kugadas - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USASudarvili Shanthalingam - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USADouglas R Call - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USADonald P Knowles - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USASubramaniam Srikumaran - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
- Publication Details
- Applied and environmental microbiology, Vol.78(18), pp.6683-6688
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Identifiers
- 99900547010001842
- Language
- English
- Resource Type
- Journal article