Journal article
Pseudechetoxin: A peptide blocker of cyclic nucleotide-gated ion channels
Proceedings of the National Academy of Sciences - PNAS, Vol.96(2), pp.754-759
01/19/1999
Handle:
https://hdl.handle.net/2376/116792
PMCID: PMC15209
PMID: 9892706
Abstract
Ion channels activated by the binding of cyclic nucleotides first were discovered in retinal rods where they generate the cell’s response to light. In other systems, however, it has been difficult to unambiguously determine whether cyclic nucleotide-dependent processes are mediated by protein kinases, their classical effector enzymes, or cyclic nucleotide-gated (CNG) ion channels. Part of this difficulty has been caused by the lack of specific pharmacological tools. Here we report the purification from the venom of the Australian King Brown snake of a peptide toxin that inhibits current through CNG channels. This toxin, which we have named Pseudechetoxin (PsTx), was purified by cation exchange and RP-HPLC and has a molecular mass of about 24 kDa. When applied to the extracellular face of membrane patches containing the α-subunit of the rat olfactory CNG channel, PsTx blocked the cGMP-dependent current with a
K
i
of 5 nM. Block was independent of voltage and required only a single molecule of toxin. PsTx also blocked CNG channels containing the bovine rod α-subunit with high affinity (100 nM), but it was less effective on the heteromeric version of the rod channel (
K
i
≈ 3 μM). We have obtained N-terminal and partial internal sequence data and the amino acid composition of PsTx. These data indicate that PsTx is a basic protein that exhibits some homology with helothermine, a toxin isolated from the venom of the Mexican beaded lizard. PsTx promises to be a valuable pharmacological tool for studies on the structure and physiology of CNG channels.
Metrics
11 Record Views
Details
- Title
- Pseudechetoxin: A peptide blocker of cyclic nucleotide-gated ion channels
- Creators
- R. Lane Brown - Neurological Sciences Institute, Oregon Health Sciences University, 1120 NW 20th Avenue, Portland, OR 97209; andTammie L Haley - Neurological Sciences Institute, Oregon Health Sciences University, 1120 NW 20th Avenue, Portland, OR 97209; andKaren A West - Neurological Sciences Institute, Oregon Health Sciences University, 1120 NW 20th Avenue, Portland, OR 97209; andJohn W Crabb - Neurological Sciences Institute, Oregon Health Sciences University, 1120 NW 20th Avenue, Portland, OR 97209; and
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.96(2), pp.754-759
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- The National Academy of Sciences
- Identifiers
- 99900547436601842
- Language
- English
- Resource Type
- Journal article