Journal article
Purification and Characterization of EDTA Monooxygenase from the EDTA-Degrading Bacterium BNC1
Journal of bacteriology, Vol.180(15), pp.3823-3827
08/1998
Handle:
https://hdl.handle.net/2376/115741
PMCID: PMC107650
PMID: 9683478
Abstract
The synthetic chelating agent EDTA can mobilize radionuclides and heavy metals in the environment. Biodegradation of EDTA should reduce this mobilization. Although several bacteria have been reported to mineralize EDTA, little is known about the biochemistry of EDTA degradation. Understanding the biochemistry will facilitate the removal of EDTA from the environment. EDTA-degrading activities were detected in cell extracts of bacterium BNC1 when flavin mononucleotide (FMN), NADH, and O
2
were present. The degradative enzyme system was separated into two different enzymes, EDTA monooxygenase and an FMN reductase. EDTA monooxygenase oxidized EDTA to glyoxylate and ethylenediaminetriacetate (ED3A), with the coconsumption of FMNH
2
and O
2
. The FMN reductase provided EDTA monooxygenase with FMNH
2
by reducing FMN with NADH. The FMN reductase was successfully substituted in the assay mixture by other FMN reductases. EDTA monooxygenase was purified to greater than 95% homogeneity and had a single polypeptide with a molecular weight of 45,000. The enzyme oxidized both EDTA complexed with various metal ions and uncomplexed EDTA. The optimal conditions for activity were pH 7.8 and 35°C.
K
m
s were 34.1 μM for uncomplexed EDTA and 8.5 μM for MgEDTA
2−
; this difference in
K
m
indicates that the enzyme has greater affinity for MgEDTA
2−
. The enzyme also catalyzed the release of glyoxylate from nitrilotriacetate and diethylenetriaminepentaacetate. EDTA monooxygenase belongs to a small group of FMNH
2
-utilizing monooxygenases that attack carbon-nitrogen, carbon-sulfur, and carbon-carbon double bonds.
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Details
- Title
- Purification and Characterization of EDTA Monooxygenase from the EDTA-Degrading Bacterium BNC1
- Creators
- Jason W Payne - Department of Microbiology, Washington State University, Pullman, Washington 99164-4233Harvey Bolton - Department of Microbiology, Washington State University, Pullman, Washington 99164-4233James A Campbell - Department of Microbiology, Washington State University, Pullman, Washington 99164-4233Luying Xun - Department of Microbiology, Washington State University, Pullman, Washington 99164-4233
- Publication Details
- Journal of bacteriology, Vol.180(15), pp.3823-3827
- Academic Unit
- Crop and Soil Sciences, Department of; Molecular Biosciences, School of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547869801842
- Language
- English
- Resource Type
- Journal article