Purification and characterization of a new β-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover

Le Gao; Feng Gao; Dongyuan Zhang; Can Zhang; Gaihong Wu; Shulin Chen

doi:10.1016/j.biortech.2013.08.089

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Purification and characterization of a new β-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover

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Purification and characterization of a new β-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover

Le Gao, Feng Gao, Dongyuan Zhang, Can Zhang, Gaihong Wu and Shulin Chen

Bioresource technology, Vol.147, pp.658-661

11/2013

DOI: https://doi.org/10.1016/j.biortech.2013.08.089

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https://hdl.handle.net/2376/118462

PMID: 24025854

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Abstract

Cellulase - metabolism

Lignin - metabolism

beta-Glucosidase - isolation & purification

Base Sequence

Electrophoresis, Polyacrylamide Gel

Substrate Specificity

beta-Glucosidase - metabolism

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Zea mays - metabolism

DNA Primers

Penicillium - enzymology

Reverse Transcriptase Polymerase Chain Reaction

A new β-glucosidase (Cel3B) was first isolated from cellulytic fungi, designated as PpCel3B. Although PpCel3B was classified to GH family 3 based on the homology sequence, PpCel3B had different biological functions in cellulose degradation and signaling molecules production. PpCel3B was constitutive and could form multiple soluble lignocellulose inducers for cellulase and hemicellulase synthesis via high tranglycosylation activity and new enzymatic activity. Moreover, PpCel3B showed apparent synergism with cellulases by removing several inhibitors. Supplementing low doses of PpCel3B (52 μg/g substrate) increased saccharification efficiency of cellulase produced by Trichoderma reesei and Penicillium piceum by 15% and 35%, respectively on delignified corn stover. PpCel3B had important application in boosting cellulase yield and efficiency.

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Title: Purification and characterization of a new β-glucosidase from Penicillium piceum and its application in enzymatic degradation of delignified corn stover
Creators: Le Gao - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
Feng Gao - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
Dongyuan Zhang - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
Can Zhang - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
Gaihong Wu - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China
Shulin Chen - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China. Electronic address: Chen_sl@tib.cas.cn
Publication Details: Bioresource technology, Vol.147, pp.658-661
Identifiers: 99900583062801842
Language: English
Resource Type: Journal article