Quantifying biofilm structure using image analysis

Xinmin Yang; Haluk Beyenal; Gary Harkin; Zbigniew Lewandowski

doi:10.1016/S0167-7012(99)00097-4

Back

Quantifying biofilm structure using image analysis

Journal article

Open access

Peer reviewed

Quantifying biofilm structure using image analysis

Xinmin Yang, Haluk Beyenal, Gary Harkin and Zbigniew Lewandowski

Journal of microbiological methods, Vol.39(2), pp.109-119

2000

DOI: https://doi.org/10.1016/S0167-7012(99)00097-4

Handle:

https://hdl.handle.net/2376/110060

PMID: 10576700

Files and links (1)

url

https://doi.org/10.1016/S0167-7012(99)00097-4View

Published (Version of record) Open

Abstract

Biofilm

Image analysis

Structure

Heterogeneity quantification

We have developed and implemented methods of extracting morphological features from images of biofilms in order to quantify the characteristics of the inherent heterogeneity. This is a first step towards quantifying the relationship between biofilm heterogeneity and the underlying processes, such as mass-transport dynamics, substrate concentrations, and species variations. We have examined two categories of features, areal, which quantify the relative magnitude of the heterogeneity and textural, which quantify the microscale structure of the heterogeneous elements. The feature set is not exhaustive and has been restricted to two-dimensional images to this point. Included in this paper are the methods used to extract the structural information and the algorithms used to quantify the data. The features discussed are porosity, fractal dimension, diffusional length, angular second moment, inverse difference moment and textural entropy. We have found that some features are better predictors of biofilm behavior than others and we discuss possible future directions for research in this area.

Metrics

8 Record Views

See more details

Details

Title: Quantifying biofilm structure using image analysis
Creators: Xinmin Yang - Center for Biofilm Engineering, P.O. Box 173980, Room 336 EPS, Montana State University, Bozeman, MT 59717, USA
Haluk Beyenal - Center for Biofilm Engineering, P.O. Box 173980, Room 336 EPS, Montana State University, Bozeman, MT 59717, USA
Gary Harkin - Computer Science Department, Montana State University, Bozeman, MT 59717, USA
Zbigniew Lewandowski - Center for Biofilm Engineering, P.O. Box 173980, Room 336 EPS, Montana State University, Bozeman, MT 59717, USA
Publication Details: Journal of microbiological methods, Vol.39(2), pp.109-119
Academic Unit: Chemical Engineering and Bioengineering, School of
Publisher: Elsevier B.V
Identifiers: 99900547119001842
Language: English
Resource Type: Journal article