Journal article
Quantitative Phosphoproteomic Analysis of Soybean Root Hairs Inoculated with Bradyrhizobium japonicum
Molecular & cellular proteomics, Vol.11(11), pp.1140-1155
11/2012
Handle:
https://hdl.handle.net/2376/101388
PMCID: PMC3494206
PMID: 22843990
Abstract
Root hairs are single hair-forming cells on roots that function to increase root surface area, enhancing water and nutrient uptake. In leguminous plants, root hairs also play a critical role as the site of infection by symbiotic nitrogen fixing rhizobia, leading to the formation of a novel organ, the nodule. The initial steps in the rhizobia-root hair infection process are known to involve specific receptor kinases and subsequent kinase cascades. Here, we characterize the phosphoproteome of the root hairs and the corresponding stripped roots (
i.e.
roots from which root hairs were removed) during rhizobial colonization and infection to gain insight into the molecular mechanism of root hair cell biology. We chose soybean (
Glycine max L
.), one of the most important crop plants in the legume family, for this study because of its larger root size, which permits isolation of sufficient root hair material for phosphoproteomic analysis. Phosphopeptides derived from root hairs and stripped roots, mock inoculated or inoculated with the soybean-specific rhizobium
Bradyrhizobium japonicum
, were labeled with the isobaric tag eight-plex iTRAQ, enriched using Ni-NTA magnetic beads and subjected to nanoRPLC-MS/MS
1
analysis using HCD and decision tree guided CID/ETD strategy. A total of 1625 unique phosphopeptides, spanning 1659 nonredundant phosphorylation sites, were detected from 1126 soybean phosphoproteins. Among them, 273 phosphopeptides corresponding to 240 phosphoproteins were found to be significantly regulated (>1.5-fold abundance change) in response to inoculation with
B. japonicum
. The data reveal unique features of the soybean root hair phosphoproteome, including root hair and stripped root-specific phosphorylation suggesting a complex network of kinase-substrate and phosphatase-substrate interactions in response to rhizobial inoculation.
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Details
- Title
- Quantitative Phosphoproteomic Analysis of Soybean Root Hairs Inoculated with Bradyrhizobium japonicum
- Creators
- Tran Hong Nha Nguyen - From the ‡Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211Laurent Brechenmacher - From the ‡Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211Joshua T Aldrich - ‖Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352Therese R Clauss - Fundamental and Computational Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352Marina A Gritsenko - Fundamental and Computational Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352Kim K Hixson - ‖Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352Marc Libault - ‡‡Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019Kiwamu Tanaka - From the ‡Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211Feng Yang - Fundamental and Computational Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352Qiuming Yao - §§Department of Computer Science, C.S. Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211Ljiljana Paša-Tolić - ‖Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352Dong Xu - §§Department of Computer Science, C.S. Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211Henry T Nguyen - From the ‡Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211Gary Stacey - From the ‡Division of Plant Sciences, C.S. Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211
- Publication Details
- Molecular & cellular proteomics, Vol.11(11), pp.1140-1155
- Academic Unit
- Plant Pathology, Department of
- Publisher
- The American Society for Biochemistry and Molecular Biology
- Grant note
- RR018522 / National Institutes of Health
- Identifiers
- 99900546633101842
- Language
- English
- Resource Type
- Journal article