Journal article
Quantitative determination of polysulfide in albumins, plasma proteins and biological fluid samples using a novel combined assays approach
Analytica chimica acta, Vol.969, pp.18-25
05/29/2017
Handle:
https://hdl.handle.net/2376/105324
PMID: 28411626
Abstract
Hydrogen sulfide (H2S) signaling involves polysulfide (RSSnSR′) formation on various proteins. However, the current lack of sensitive polysulfide detection assays poses methodological challenges for understanding sulfane sulfur homeostasis and signaling. We developed a novel combined assay by modifying Sulfide Antioxidant Buffer (SAOB) to produce an “Elimination Method of Sulfide from Polysulfide” (EMSP) treatment solution that liberates sulfide, followed with methylene blue (MB) sulfide detection assay. The combined EMSP-MB sulfide detection assay performed on low molecular weight sulfur species showed that sulfide was produced from trisulfide compounds such as glutathione trisulfide and diallyl trisulfide, but not from the thiol compounds such as cysteine, cystine and glutathione. In the case of plasma proteins, this novel combined detection assay revealed that approximately 14.7, 1.7, 3.9, 3.7 sulfide mol/mol released from human serum albumin, α1-anti-trypsin, α1-acid glycoprotein and ovalbumin, respectively, suggesting that serum albumin is a major pool of polysulfide in human blood circulation. Taken together with the results of albumins of different species, the liberated sulfide has a good correlation with cysteine instead of methionine, indicating the site of incorporation of polysulfide is cysteine. With this novel sulfide detention assay, approximately 8,000, 120 and 1100 μM of polysulfide concentrations was quantitated in human healthy plasma, saliva and tear, respectively. Our promising polysulfide specific detection assay can be a very important tool because quantitative determination of polysulfide sheds light on the functional consequence of protein-bound cysteine polysulfide and expands the research area of reactive oxygen to reactive polysulfide species.
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•Reactive sulfur species like Polysulfide behaves as potent antioxidants and redox signaling intermediates.•We developed a simple method of sulfide eliminated from polysulfide.•We found that serum albumin is pool of polysulfide in human blood circulation.•With this method, we could quantify polysulfide concentrations in various human fluids.
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Details
- Title
- Quantitative determination of polysulfide in albumins, plasma proteins and biological fluid samples using a novel combined assays approach
- Creators
- Mayumi Ikeda - Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, JapanYu Ishima - Department of Pharmacokinetics and Biopharmaceutics, Institute of Biomedical Sciences, Tokushima University, 1-78-1, Sho-machi, Tokushima 770-8505, JapanAkitomo Shibata - Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, JapanVictor T.G Chuang - School of Pharmacy, Curtin University, Perth 6845, Western Australia, AustraliaTomohiro Sawa - Department of Microbiology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556, JapanHideshi Ihara - Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Osaka 599-8531, JapanHiroshi Watanabe - Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, JapanMing Xian - Department of Chemistry, Washington State University, Pullman, WA 99164, United StatesYuya Ouchi - Dojindo Laboratories, Kumamoto, 2025-5 Tahara, Mashikimachi, Kamimashikigun, 861-2202, JapanTaro Shimizu - Department of Pharmacokinetics and Biopharmaceutics, Institute of Biomedical Sciences, Tokushima University, 1-78-1, Sho-machi, Tokushima 770-8505, JapanHidenori Ando - Department of Pharmacokinetics and Biopharmaceutics, Institute of Biomedical Sciences, Tokushima University, 1-78-1, Sho-machi, Tokushima 770-8505, JapanMasami Ukawa - Department of Pharmacokinetics and Biopharmaceutics, Institute of Biomedical Sciences, Tokushima University, 1-78-1, Sho-machi, Tokushima 770-8505, JapanTatsuhiro Ishida - Department of Pharmacokinetics and Biopharmaceutics, Institute of Biomedical Sciences, Tokushima University, 1-78-1, Sho-machi, Tokushima 770-8505, JapanTakaaki Akaike - Department of Environmental Health Sciences and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai 980-8575, JapanMasaki Otagiri - Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, JapanToru Maruyama - Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan
- Publication Details
- Analytica chimica acta, Vol.969, pp.18-25
- Academic Unit
- Chemistry, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546633601842
- Language
- English
- Resource Type
- Journal article