Journal article
RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells
The Plant cell, Vol.30(10), pp.2529-2552
10/2018
Handle:
https://hdl.handle.net/2376/107297
PMCID: PMC6241268
PMID: 30190374
Abstract
In developing rice (
) endosperm, mRNAs of the major storage proteins, glutelin and prolamine, are transported and anchored to distinct subdomains of the cortical endoplasmic reticulum. RNA binding protein RBP-P binds to both glutelin and prolamine mRNAs, suggesting a role in some aspect of their RNA metabolism. Here, we show that rice lines expressing mutant RBP-P mislocalize both glutelin and prolamine mRNAs. Different mutant RBP-P proteins exhibited varying degrees of reduced RNA binding and/or protein-protein interaction properties, which may account for the mislocalization of storage protein RNAs. In addition, partial loss of RBP-P function conferred a broad phenotypic variation ranging from dwarfism, chlorophyll deficiency, and sterility to late flowering and low spikelet fertility. Transcriptome analysis highlighted the essential role of RBP-P in regulating storage protein genes and several essential biological processes during grain development. Overall, our data demonstrate the significant roles of RBP-P in glutelin and prolamine mRNA localization and in the regulation of genes important for plant growth and development through its RNA binding activity and cooperative regulation with interacting proteins.
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Details
- Title
- RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells
- Creators
- Li Tian - Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340Hong-Li Chou - Center for RNA Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802Laining Zhang - Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340Seon-Kap Hwang - Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340Shawn R Starkenburg - Los Alamos National Laboratory, Los Alamos, New Mexico 87545Kelly A Doroshenk - Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340Toshihiro Kumamaru - Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, JapanThomas W Okita - Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340 okita@wsu.edu
- Publication Details
- The Plant cell, Vol.30(10), pp.2529-2552
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- United States
- Identifiers
- 99900546902301842
- Language
- English
- Resource Type
- Journal article