Rac interacts with Abi-1 and WAVE2 to promote an Arp2/3-dependent actin recruitment during chlamydial invasion

Rey A Carabeo; Cheryl A Dooley; Scott S Grieshaber; Ted Hackstadt

doi:10.1111/j.1462-5822.2007.00958.x

Back

Rac interacts with Abi-1 and WAVE2 to promote an Arp2/3-dependent actin recruitment during chlamydial invasion

Journal article

Peer reviewed

Rac interacts with Abi-1 and WAVE2 to promote an Arp2/3-dependent actin recruitment during chlamydial invasion

Rey A Carabeo, Cheryl A Dooley, Scott S Grieshaber and Ted Hackstadt

Cellular microbiology, Vol.9(9), pp.2278-2288

09/2007

DOI: https://doi.org/10.1111/j.1462-5822.2007.00958.x

Handle:

https://hdl.handle.net/2376/115908

PMID: 17501982

Abstract

RNA, Small Interfering - genetics

Wiskott-Aldrich Syndrome Protein Family - metabolism

Cytoskeletal Proteins - genetics

Humans

Actins - metabolism

Cercopithecus aethiops

rac GTP-Binding Proteins - metabolism

Actin-Related Protein 2-3 Complex - genetics

Chlamydia trachomatis - metabolism

Recombinant Fusion Proteins - metabolism

Actin-Related Protein 2-3 Complex - metabolism

Animals

rac GTP-Binding Proteins - genetics

Adaptor Proteins, Signal Transducing - genetics

Chlamydia trachomatis - pathogenicity

Recombinant Fusion Proteins - genetics

Cytoskeletal Proteins - metabolism

Enzyme Activation

HeLa Cells

Adaptor Proteins, Signal Transducing - metabolism

Wiskott-Aldrich Syndrome Protein Family - genetics

COS Cells

Chlamydia Infections - metabolism

RNA, Small Interfering - metabolism

Chlamydiae are Gram-negative obligate intracellular pathogens to which access to an intracellular environment is fundamental to their development. Chlamydial attachment to host cells induces the activation of the Rac GTPase, which is required for the localization of WAVE2 at the sites of chlamydial entry. Co-immunoprecipitation experiments demonstrated that Chlamydia trachomatis infection promoted the interaction of Rac with WAVE2 and Abi-1, but not with IRSp53. siRNA depletion of WAVE2 and Abi-1 abrogated chlamydia-induced actin recruitment and significantly reduced the uptake of the pathogen by the depleted cells. Chlamydia invasion also requires the Arp2/3 complex as demonstrated by its localization to the sites of chlamydial attachment and the reduced efficiency of chlamydial invasion in cells overexpressing the VCA domain of the neural Wiskott-Aldrich syndrome protein. Thus, C. trachomatis activates Rac and promotes its interaction with WAVE2 and Abi-1 to activate the Arp2/3 complex resulting in the induction of actin cytoskeletal rearrangements that are required for invasion.

Metrics

15 Record Views

Details

Title: Rac interacts with Abi-1 and WAVE2 to promote an Arp2/3-dependent actin recruitment during chlamydial invasion
Creators: Rey A Carabeo - Department of Microbiology and Immunology, University of Louisville School of Medicine, Louisville, KY, USA
Cheryl A Dooley
Scott S Grieshaber
Ted Hackstadt
Publication Details: Cellular microbiology, Vol.9(9), pp.2278-2288
Academic Unit: Molecular Biosciences, School of
Publisher: England
Grant note: R01 AI065545-01 / NIAID NIH HHS R01 AI065545 / NIAID NIH HHS AI065545 / NIAID NIH HHS Intramural NIH HHS K22 AI052252-02 / NIAID NIH HHS K22 AI052252 / NIAID NIH HHS
Identifiers: 99900547912101842
Language: English
Resource Type: Journal article

Rac interacts with Abi-1 and WAVE2 to promote an Arp2/3-dependent actin recruitment during chlamydial invasion

Related links

Abstract

Metrics

Details