Journal article
Rad23 is required for transcription-coupled repair and efficient overall repair in Saccharomyces cerevisiae
Molecular and cellular biology, Vol.16(5), pp.2361-2368
01/01/1996
Handle:
https://hdl.handle.net/2376/102585
PMID: 8628303
Abstract
The repair of UV-induced photoproducts (cyclobutane pyrimidine dimers) in a well-characterized minichromosome, genomic DNA, and a transcribed genomic gene (RPB2) of a rad23 Delta mutant of Saccharomyces cerevisiae was examined. Isogenic wild-type cells show a strong bias for the repair of the transcribed strands in both the plasmid and genomic genes and efficient overall repair of both DNAs (>80% of the dimers were removed in 6 h). However, the rad23 Delta mutant shows (i) no strand bias for repair in these genes and decreased repair of both strands, (ii) partial repair of genomic DNA ( similar to 45% in 6 h), and (iii) very poor repair of the plasmid overall ( similar to 15% in 6 h). These features, coupled with the decreased UV survival of rad23 Delta cells, indicate that Rad23 is required for both transcription-coupled repair and efficient overall repair in S. cerevisiae.
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Details
- Title
- Rad23 is required for transcription-coupled repair and efficient overall repair in Saccharomyces cerevisiae
- Creators
- J P MuellerM J Smerdon
- Publication Details
- Molecular and cellular biology, Vol.16(5), pp.2361-2368
- Academic Unit
- Molecular Biosciences, School of
- Identifiers
- 99900546521701842
- Language
- English
- Resource Type
- Journal article