Journal article
Rad26, the Transcription-Coupled Repair Factor in Yeast, Is Required for Removal of Stalled RNA Polymerase-II following UV Irradiation
PloS one, Vol.8(8), pp.e72090-e72090
08/21/2013
Handle:
https://hdl.handle.net/2376/110757
PMCID: PMC3749123
PMID: 23991048
Abstract
Transcription coupled nucleotide excision repair (TCR) is a major pathway responsible for removal of helix distorting DNA lesions from transcriptionally active regions of the genome. Rad26, a key factor of the TCR pathway, is known to play a role during early steps of TCR. Here, we show that Rad26-mediated TCR is not absolutely dependent on active transcription elongation in budding yeast. As per our results,
RAD26-
deleted cells show enhanced UV sensitivity compared to wild type cells under conditions where transcription elongation is inhibited. The increased UV sensitivity observed in
RAD26
-deleted cells, however, is not due to reduced expression of the major NER-responsive genes. Interestingly, transcription of the constitutively expressed
RPB2
gene is adversely affected in
RAD26-
deleted cells during UV-induced DNA damage repair. In consonance, chromatin immunoprecipitation analysis showed that unlike in wild type, in
RAD26
-deleted cells no significant reduction in RNA polymerase II occupancy occurs during nucleotide excision repair in the transcriptionally active loci like,
RPB2
,
PYK1
and
RPL2B
. These results collectively indicate that removal of RNAPII during DNA damage repair following UV irradiation is dependent on Rad26.
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Details
- Title
- Rad26, the Transcription-Coupled Repair Factor in Yeast, Is Required for Removal of Stalled RNA Polymerase-II following UV Irradiation
- Creators
- Sounak Ghosh-RoyDhiman DasDebarati ChowdhuryMichael J.SmerdonRonita Nag Chaudhuri
- Publication Details
- PloS one, Vol.8(8), pp.e72090-e72090
- Publisher
- Public Library of Science; San Francisco, USA
- Identifiers
- 99900547176901842
- Language
- English
- Resource Type
- Journal article