Journal article
Rapid Detection and Monitoring of Flavobacterium psychrophilum in Water by Using a Handheld, Field-Portable Quantitative PCR System
Journal of aquatic animal health, Vol.30(4), pp.302-311
12/2018
Handle:
https://hdl.handle.net/2376/112740
PMID: 30269364
Abstract
Advances in technology are making it easier for rapid field detection of microbes in aquaculture. Specifically, real-time quantitative PCR (qPCR) analysis, which has traditionally been confined to laboratory-based protocols, is now available in a handheld, field-portable system. The feasibility of using the Biomeme handheld qPCR system for rapid (<50 min) on-site detection and monitoring of Flavobacterium psychrophilum from filtered water samples was evaluated. Paired water samples were collected over a 23-d period from microcosm tanks that housed fish injected with known levels of F. psychrophilum. Water samples were filtered through 0.45-μm nitrocellulose filters and were analyzed with both the Biomeme qPCR platform and a traditional bench qPCR protocol. The two methods identified similar fluctuations in F. psychrophilum DNA throughout the study. Standard curves relating quantification cycles to the number of F. psychrophilum colony-forming units (CFU) were constructed and analyzed; results indicated that CFU increased rapidly between days 6 and 8 of the trial and then progressively decreased during the remaining 15 d. Average calculated log
(CFU/mL) values were significantly correlated between the two platforms. Rapid, field-based qPCR can be incorporated into daily water quality monitoring protocols to help detect and monitor microbes in aquaculture systems.
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Details
- Title
- Rapid Detection and Monitoring of Flavobacterium psychrophilum in Water by Using a Handheld, Field-Portable Quantitative PCR System
- Creators
- Phong L Nguyen - Smith-Root, Inc., 16603 Northeast 50th Avenue, Vancouver, Washington, 98686, USAPonnerassery S Sudheesh - Florida Department of Agriculture and Consumer Services, Bronson Animal Disease Diagnostic Laboratory, 2700 North John Young Parkway, Kissimmee, Florida, 34741, USAAusten C Thomas - Smith-Root, Inc., 16603 Northeast 50th Avenue, Vancouver, Washington, 98686, USAMieke Sinnesael - Biomeme, 1015 Chestnut Street, Suite 1401, Philadelphia, Pennsylvania, 19107, USAKatherine Haman - Syndel, 1441 Smith Road, Ferndale, Washington, 98248, USAKenneth D Cain - Department of Fish and Wildlife Sciences and Aquaculture Research Institute, University of Idaho, 875 Perimeter Drive, Moscow, Idaho, 83844, USA
- Publication Details
- Journal of aquatic animal health, Vol.30(4), pp.302-311
- Academic Unit
- Center for Reproductive Biology
- Publisher
- United States
- Identifiers
- 99900547403801842
- Language
- English
- Resource Type
- Journal article