Journal article
Rapid and sensitive detection of microRNA via the capture of fluorescent Ayes-loaded albumin nangparticles around functionalized magnetic beads
Biosensors & bioelectronics, Vol.94, pp.56-62
08/15/2017
PMID: 28257975
Abstract
MicroRNAs (miRNAs) play important roles in gene regulation and cancer development. Nowadays, it is still a challenge to detect low-abundance miRNAs. Here, we present a magnetic fluorescent miRNA sensing system for the rapid and sensitive detection of miRNAs from cell lysates and serum samples. In this system, albumin nanoparticles (Alb NPs) were prepared from inherent biocompatible bovine serum albumin (BSA). A large number of fluorescent dyes were loaded into Alb NPs to make Alb NPs serve as signal molecular nanocarriers for signal amplification. Benefited from the reactive functional groups-carboxyl groups of Alb NPs, p19 protein, a viral protein that can bind and sequester short RNA duplex effectively and selectively, was modified successfully to the surface of the fluorescent dyes-loaded Alb NPs, thus enabling the probe:target miRNA duplex recognition and binding. Followed by the introduction of gold nanoparticles coated magnetic microbeads (Au NPs-MBs), which were prepared through a novel and simple method, the system combined the merits of the rapid and efficient collection given by MBs with the good affinities to attach probe molecules endowed by the coated gold layer. A broad linear detection range of 10 fM-10 nM and a low detection limit of 9 fM were obtained within 100 min by detecting a model target miRNA-21. The feasibility of this method for rapid and sensitive quantification might advance the use of miRNAs as biomarkers in clinical praxis significantly.
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Details
- Title
- Rapid and sensitive detection of microRNA via the capture of fluorescent Ayes-loaded albumin nangparticles around functionalized magnetic beads
- Creators
- Tianxiang Wei - Nanjing Normal UniversityDan Du - Washington State UniversityZhaoyin Wang - Nanjing Normal UniversityWeiwei Zhang - Nanjing Normal UniversityYuehe Lin - Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USAZhihui Dai - Nanjing Normal University
- Publication Details
- Biosensors & bioelectronics, Vol.94, pp.56-62
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier
- Number of pages
- 7
- Grant note
- 201406860004 / China Scholarship Council Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions 21625502; 21475062 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC)
- Identifiers
- 99901227635001842
- Language
- English
- Resource Type
- Journal article