Rapid and targeted introgression of genes into popular wheat cultivars using marker-assisted background selection

Harpinder S Randhawa; Jasdeep S Mutti; Kim Kidwell; Craig F Morris; Xianming Chen; Kulvinder S Gill

doi:10.1371/journal.pone.0005752

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Rapid and targeted introgression of genes into popular wheat cultivars using marker-assisted background selection

Harpinder S Randhawa, Jasdeep S Mutti, Kim Kidwell, Craig F Morris, Xianming Chen and Kulvinder S Gill

PloS one, Vol.4(6), pp.e5752-e5752

06/01/2009

DOI: https://doi.org/10.1371/journal.pone.0005752

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https://hdl.handle.net/2376/103507

PMCID: PMC2684585

PMID: 19484121

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Abstract

Genes, Plant

Probability

Genotype

Chromosome Mapping

Genetic Markers

Genome, Plant

Triticum - genetics

Chromosomes - ultrastructure

Recombination, Genetic

Computer Simulation

Heterozygote

Plant Diseases - genetics

Models, Genetic

Crosses, Genetic

A marker-assisted background selection (MABS)-based gene introgression approach in wheat (Triticum aestivum L.) was optimized, where 97% or more of a recurrent parent genome (RPG) can be recovered in just two backcross (BC) generations. A four-step MABS method was developed based on 'Plabsim' computer simulations and wheat genome structure information. During empirical optimization of this method, double recombinants around the target gene were selected in a step-wise fashion during the two BC cycles followed by selection for recurrent parent genotype on non-carrier chromosomes. The average spacing between carrier chromosome markers was <4 cM. For non-carrier chromosome markers that flanked each of the 48 wheat gene-rich regions, this distance was approximately 12 cM. Employed to introgress seedling stripe rust (Puccinia striiformis f. sp. tritici) resistance gene Yr15 into the spring wheat cultivar 'Zak', marker analysis of 2,187 backcross-derived progeny resulted in the recovery of a BC(2)F(2ratio3) plant with 97% of the recurrent parent genome. In contrast, only 82% of the recurrent parent genome was recovered in phenotypically selected BC(4)F(7) plants developed without MABS. Field evaluation results from 17 locations indicated that the MABS-derived line was either equal or superior to the recurrent parent for the tested agronomic characteristics. Based on these results, MABS is recommended as a strategy for rapidly introgressing a targeted gene into a wheat genotype in just two backcross generations while recovering 97% or more of the recurrent parent genotype.

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Title: Rapid and targeted introgression of genes into popular wheat cultivars using marker-assisted background selection
Creators: Harpinder S Randhawa - Department of Crop and Soil Sciences, Washington State University, Pullman, WA, USA
Jasdeep S Mutti
Kim Kidwell
Craig F Morris
Xianming Chen
Kulvinder S Gill
Publication Details: PloS one, Vol.4(6), pp.e5752-e5752
Academic Unit: Crop and Soil Sciences, Department of; Plant Pathology, Department of
Publisher: United States
Identifiers: 99900546504201842
Language: English
Resource Type: Journal article