Journal article
Rapid detection of dysfunctional high‐density lipoproteins using isoelectric focusing‐based microfluidic device to diagnose senescence‐related disease
Electrophoresis, Vol.32(23), pp.3415-3423
11/2011
Handle:
https://hdl.handle.net/2376/106297
PMID: 22134980
Abstract
Recently, we reported elevated levels of advanced glycated end products (AGEs) in human high‐density lipoproteins (HDL), with fragmentation of apoA‐I in an elderly group, compared with a younger group. More dysfunctional HDL from human plasma was demonstrated in the elderly group, including reconstituted HDL containing glycated apoA‐I (gA‐I‐rHDL) with elevation of AGEs. Based on SDS‐PAGE analysis, HDL3 from the elderly group (E‐HDL3) exhibited increased multimerization with increased smear band intensity compared to HDL3 from the younger group (Y‐HDL3). According to isoelectric focusing gel analysis, gA‐I‐rHDL and E‐HDL3 showed electromobility to the basic region of pH with a broader band range. In a microfluidic channel, E‐HDL3 had faster mobility with a broader range and a higher isoelectric point (pI, approximately 8.1), whereas Y‐HDL3 showed a narrow band range with a lower pI (approximately 6.9). In conclusion, gA‐I‐rHDL and E‐HDL share several electrophoretic properties with multimerization and faster mobility in microfluidic channels, depending on the isoelectric point. These results can be applied to develop a rapid detection system for modified HDL to predict the extent of aging and aging‐related metabolic diseases, such as cardiovascular disease and diabetes.
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Details
- Title
- Rapid detection of dysfunctional high‐density lipoproteins using isoelectric focusing‐based microfluidic device to diagnose senescence‐related disease
- Creators
- Wookju JangJaesool ShimDong‐Yeon LeePrashanta DuttaJae‐Ryong KimKyung‐Hyun Cho
- Publication Details
- Electrophoresis, Vol.32(23), pp.3415-3423
- Academic Unit
- Mechanical and Materials Engineering, School of
- Publisher
- WILEY‐VCH Verlag; Weinheim
- Number of pages
- 9
- Grant note
- National Research Foundation of Korea (NRF) Aging‐associated Vascular Disease Research Center (R13‐2005‐005‐01003‐0 [2011]) Basic Science Researcher Program (2010‐0020910) Mid‐career Researcher Program (2011‐0015529)
- Identifiers
- 99900546896801842
- Language
- English
- Resource Type
- Journal article