Journal article
Rapid determination of triazophos using acetylcholinesterase biosensor based on sol–gel interface assembling multiwall carbon nanotubes
Journal of applied electrochemistry, Vol.37(8), pp.893-898
08/2007
Handle:
https://hdl.handle.net/2376/111634
Abstract
A sensitive and stable amperometric sensor has been devised for rapid determination of triazophos based on efficient immobilization of acetylcholinesterase (AChE) on silica sol–gel (SiSG) film assembling multiwall carbon nanotubes (MWNTs). The sol–gel matrix provided a biocompatible microenvironment around the enzyme and efficiently prevented leakage of the enzyme from the film. In the presence of acetylthiocholine chloride (ATCl) as a substrate, MWNTs promoted electron transfer reactions at a lower potential and catalyzed electrochemical oxidation of enzymatically formed thiocholine, thus increasing detection sensitivity. Based on the inhibition of organophosphorous compound on the enzymatic activity of AChE, using triazophos as a model compound, the effects of pH, temperature, and MWNTs contents were explored. Under optimum conditions, the inhibition of triazophos was proportional to its concentration from 0.02 μM to 1 μM and from 5 μM to 30 μM, with a detection limit of 0.005 μM. The determination of triazophos in garlic samples showed acceptable accuracy. Fabrication reproducibility of the sensor was good and stability was acceptable. The sensor is a promising new tool for pesticide analysis.
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Details
- Title
- Rapid determination of triazophos using acetylcholinesterase biosensor based on sol–gel interface assembling multiwall carbon nanotubes
- Creators
- Dan Du - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education Central China Normal University Wuhan 430079 P.R. ChinaJie Cai - The Technology Center of Wuhan Iron & Steel Company Wuhan 430080 P.R. ChinaDandan Song - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education Central China Normal University Wuhan 430079 P.R. ChinaAidong Zhang - Key Laboratory of Pesticide & Chemical Biology of Ministry of Education Central China Normal University Wuhan 430079 P.R. China
- Publication Details
- Journal of applied electrochemistry, Vol.37(8), pp.893-898
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Kluwer Academic Publishers; Dordrecht
- Identifiers
- 99900547602301842
- Language
- English
- Resource Type
- Journal article