Journal article
Rapid separation of phenylthiohydantoin amino acids: ambient pressure ion-mobility mass spectrometry (IMMS)
Analytical and bioanalytical chemistry, Vol.375(1), pp.99-102
01/2003
Handle:
https://hdl.handle.net/2376/110058
PMID: 12520443
Abstract
An electrospray ionization (ESI) ambient pressure ion-mobility spectrometer (APIMS) interfaced to an orthogonal reflector time-of-flight mass spectrometer (TOFMS) was evaluated for the first time as a detector for the identification of phenylthiohydantoin (PTH)-derivatized amino acids, the final products in the Edman sequencing process of peptides and proteins. The drift and flight times of the twenty common PTH amino acids were characterized by a well-defined 2-D mobility/mass spectral pattern. The combination of mobility/mass modes of analysis gave rise to a unique trend-line formation for the series of PTH amino acids. In addition, each PTH amino acid had a unique reduced mobility constant Ko, thus enabling the differentiation of all the amino acid derivatives including the PTH-leucine and PTH-isoleucine isomers. More importantly it was shown that it was possible to resolve a complete reference mixture of PTH amino acids in a single experimental run in less than 1 min. Detection limits for the PTH amino acids were found to range from 1.04 to 3.52 ng; indicating that the limits of detection were less than 17.0 pmol for all of the PTH amino acids.
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Details
- Title
- Rapid separation of phenylthiohydantoin amino acids: ambient pressure ion-mobility mass spectrometry (IMMS)
- Creators
- Wes Steiner - Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USABrian Clowers - Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USAHerbert Hill - Department of Chemistry, Washington State University, Pullman, WA 99164-4630, USA
- Publication Details
- Analytical and bioanalytical chemistry, Vol.375(1), pp.99-102
- Academic Unit
- Chemistry, Department of
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900547196501842
- Language
- English
- Resource Type
- Journal article