Journal article
Rapid triacylglyceride detection and quantification in live micro-algal cultures via liquid state 1H NMR
Algal research (Amsterdam), Vol.1(2), pp.166-175
10/2012
Handle:
https://hdl.handle.net/2376/118288
Abstract
Non-invasive methods for measuring lipid content in live microalgal cultures are critically needed for algal biofuel research and development. A non-destructive method requiring minimal sample preparation was developed utilizing liquid state ¹H NMR for quantifying triacylglycerides (TAGs) in live algae cultures. ¹H NMR and ¹H HR-MAS spectra of live algae cells show outstanding correlation with published chemical shifts for TAGs. ¹H NMR spectra of Chlamydomonas reinhardtii whole cells and isolated lipid bodies were compared with a standard oleic TAG ¹H spectrum, demonstrating that only lipid body TAGs were observed. A “model” TAG was derived, providing a proton count and molecular weight for conversion of TAG ¹H NMR integrals to volumetric TAG or fatty acid methyl ester (FAME) equivalent concentrations, which were correlated to FAME concentrations by gas chromatography (FAME-GC) at several time points. A customized NMR flow cell was subsequently constructed, allowing real-time, continuous measurements of multiple cultures.
► ¹H NMR method for detection of triacylglycerides in live microalgal cultures. ► Quantitation model TAG includes weighted average MW and proton count. ► ¹H NMR and FAME-GC data correlated throughout a growth cycle. ► A flow NMR probe was constructed to provide real-time TAG measurements.
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Details
- Title
- Rapid triacylglyceride detection and quantification in live micro-algal cultures via liquid state 1H NMR
- Creators
- Peter T Davey - Biological Systems Engineering, PO Box 646120, Washington State University, Pullman, WA 99164–6120, USAWilliam C Hiscox - Center for NMR Spectroscopy, PO Box 644630, Washington State University, Pullman, WA 99164–4630, USABen F Lucker - Biological Systems Engineering, PO Box 646120, Washington State University, Pullman, WA 99164–6120, USAJames V O'Fallon - Biological Systems Engineering, PO Box 646120, Washington State University, Pullman, WA 99164–6120, USAShulin Chen - Biological Systems Engineering, PO Box 646120, Washington State University, Pullman, WA 99164–6120, USAGregory L Helms - Center for NMR Spectroscopy, PO Box 644630, Washington State University, Pullman, WA 99164–4630, USA
- Publication Details
- Algal research (Amsterdam), Vol.1(2), pp.166-175
- Publisher
- Elsevier B.V
- Identifiers
- 99900583057201842
- Language
- English
- Resource Type
- Journal article