Journal article
Rational Design of a Dual-Reactivity-Based Fluorescent Probe for Visualizing Intracellular HSNO
Angewandte Chemie (International ed.), Vol.58(45), pp.16067-16070
11/04/2019
Handle:
https://hdl.handle.net/2376/112956
PMID: 31479578
Abstract
Thionitrous acid (HSNO), the smallest S-nitrosothiol, is emerging as a potential key intermediate in cellular redox regulation linking two signaling molecules H
S and NO. However, the chemical biology of HSNO remains poorly understood. A major hurdle is the lack of methods for selective detection of HSNO in biological systems. Herein, we report the rational design, synthesis, and evaluation of the first fluorescent probe TAP-1 for HSNO detection. TAP-1 showed high selectivity and sensitivity to HSNO in aqueous media and cells, providing a useful tool for understanding the functions of HSNO in biology.
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Details
- Title
- Rational Design of a Dual-Reactivity-Based Fluorescent Probe for Visualizing Intracellular HSNO
- Creators
- Wei Chen - Department of Chemistry, Washington State University, Pullman, WA, 99164, USATetsuro Matsunaga - Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, 980-8575, JapanDeshka L Neill - Department of Chemistry, Washington State University, Pullman, WA, 99164, USAChun-Tao Yang - Department of Chemistry, Washington State University, Pullman, WA, 99164, USATakaaki Akaike - Department of Environmental Medicine and Molecular Toxicology, Tohoku University Graduate School of Medicine, Sendai, 980-8575, JapanMing Xian - Department of Chemistry, Washington State University, Pullman, WA, 99164, USA
- Publication Details
- Angewandte Chemie (International ed.), Vol.58(45), pp.16067-16070
- Academic Unit
- Chemistry, Department of
- Publisher
- Germany
- Grant note
- 19K07554 / Ministry of Education, Culture, Sports, Science and Technology 18H05277 / Ministry of Education, Culture, Sports, Science and Technology CHE1738305 / National Science Foundation R01 GM125968 / NIGMS NIH HHS
- Identifiers
- 99900547535501842
- Language
- English
- Resource Type
- Journal article