Journal article
Reactive oxygen species-triggered off-on fluorescence donor for imaging hydrogen sulfide delivery in living cells† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c9sc02323b
Chemical science (Cambridge), Vol.10(33), pp.7690-7694
07/01/2019
Handle:
https://hdl.handle.net/2376/109426
PMCID: PMC6836935
PMID: 31803407
Abstract
A reactive oxygen species-triggered off-on fluorescence H
2
S donor is develop for the real-time imaging of H
2
S delivery and the cytoprotection against the hazardous oxidative environment.
Hydrogen sulfide (H
2
S), an important gasotransmitter, can mediate a variety of pathophysiological processes, and H
2
S-based donors have been intensively explored for the therapy of cardiovascular injury, nerve damage and intestinal disorders. However, most of the H
2
S donors are not capable of simultaneously real-time tracking intracellular H
2
S delivery, which limits their biological application for elucidating the specific function of H
2
S. Herein we develop the first reactive oxygen species (ROS)-triggered off-on fluorescence H
2
S donor (NAB) by incorporating ROS-responsive arylboronate into a fluorophore through thiocarbamate. The donor NAB can release carbonyl sulfide (COS) and the fluorophore with a fluorescence off-on response
via
a ROS-triggered self-immolative reaction, and then COS is quickly converted to H
2
S by the ubiquitous carbonic anhydrase. This dual function makes NAB suitable for not only
in situ
and real-time monitoring of the intracellular H
2
S release but also rescuing RAW264.7 cells from the hazardous oxidative environment under the stimulation of phorbol-12-myristate-13-acetate, revealing the possible potential of NAB as a therapeutic prodrug with the fluorescence imaging capacity.
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Details
- Title
- Reactive oxygen species-triggered off-on fluorescence donor for imaging hydrogen sulfide delivery in living cells† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c9sc02323b
- Creators
- Yiming Hu - Beijing National Laboratory for Molecular Sciences , Key Laboratory of Analytical Chemistry for Living Biosystems , Institute of Chemistry , Chinese Academy of Sciences , Beijing 100190 , China . EmailXiaoyi Li - Beijing National Laboratory for Molecular Sciences , Key Laboratory of Analytical Chemistry for Living Biosystems , Institute of Chemistry , Chinese Academy of Sciences , Beijing 100190 , China . EmailYu Fang - Beijing National Laboratory for Molecular Sciences , Key Laboratory of Analytical Chemistry for Living Biosystems , Institute of Chemistry , Chinese Academy of Sciences , Beijing 100190 , China . EmailWen Shi - Beijing National Laboratory for Molecular Sciences , Key Laboratory of Analytical Chemistry for Living Biosystems , Institute of Chemistry , Chinese Academy of Sciences , Beijing 100190 , China . EmailXiaohua Li - Beijing National Laboratory for Molecular Sciences , Key Laboratory of Analytical Chemistry for Living Biosystems , Institute of Chemistry , Chinese Academy of Sciences , Beijing 100190 , China . EmailWei Chen - Department of Chemistry , Washington State University , Pullman , Washington 99164 , USAMing Xian - Department of Chemistry , Washington State University , Pullman , Washington 99164 , USAHuimin Ma - Beijing National Laboratory for Molecular Sciences , Key Laboratory of Analytical Chemistry for Living Biosystems , Institute of Chemistry , Chinese Academy of Sciences , Beijing 100190 , China . Email
- Publication Details
- Chemical science (Cambridge), Vol.10(33), pp.7690-7694
- Academic Unit
- Chemistry, Department of
- Publisher
- Royal Society of Chemistry
- Identifiers
- 99900547017101842
- Language
- English
- Resource Type
- Journal article