Journal article
Recessive-interfering mutations in the gibberellin signaling gene SLEEPY1 are rescued by overexpression of its homologue, SNEEZY
Proceedings of the National Academy of Sciences - PNAS, Vol.101(34), pp.12771-12776
08/24/2004
Handle:
https://hdl.handle.net/2376/105708
PMCID: PMC515128
PMID: 15308775
Abstract
This article reports the genetic interaction of two F-box genes,
SLEEPY1
(
SLY1
) and
SNEEZY
(
SNE
), in
Arabidopsis thaliana
gibberellin (GA) signaling. The
SLY1
gene encodes an F-box subunit of a Skp1-cullin-F-box (SCF) E3 ubiquitin ligase complex that positively regulates GA signaling. The
sly1-2
and
sly1-10
mutants have recessive, GA-insensitive phenotypes including delayed germination, dwarfism, reduced fertility, and overaccumulation of the DELLA proteins RGA (Repressor of ga1-3), GAI (GA-Insensitive), and RGL2 (RGA-Like 2). The DELLA domain proteins are putative transcription factors that negatively regulate GA signaling. The requirement for
SLY1
in GA-stimulated disappearance of DELLA proteins suggests that GA targets DELLA proteins for destruction via SCF
SLY1
-mediated ubiquitylation. Overexpression of
SLY1
in
sly1-2
and
sly1-10
plants rescues the recessive GA-insensitive phenotype of these mutants. Surprisingly, antisense expression of
SLY1
also suppresses these mutants. This result caused us to hypothesize that the
SLY1
homologue
SNE
can functionally replace
SLY1
in the absence of the recessive interfering
sly1-2
or
sly1-10
genes. This hypothesis was supported because overexpression of
SNE
in
sly1-10
rescues the dwarf phenotype. In addition to rescuing the
sly1-10
dwarf phenotype,
SNE
overexpression also restored normal RGA protein levels, suggesting that the SNE F-box protein can replace SLY1 in the GA-induced proteolysis of RGA. If the C-terminal truncation in the
sly1-2
and
sly1-10
alleles interferes with
SNE
rescue, we reasoned that overexpression of
sly1-2
might interfere with wild-type
SLY1
function. Indeed, overexpression of
sly1-2
in wild-type L
er
(Landsberg
erecta
) yields dwarf plants.
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Details
- Title
- Recessive-interfering mutations in the gibberellin signaling gene SLEEPY1 are rescued by overexpression of its homologue, SNEEZY
- Creators
- Lucia C Strader - U.S. Department of Agriculture-Agricultural Research Service and Department of Crop and Soil Science and Graduate Program in Molecular Plant Sciences, Washington State University, Pullman, WA 99164-6420Siân Ritchie - U.S. Department of Agriculture-Agricultural Research Service and Department of Crop and Soil Science and Graduate Program in Molecular Plant Sciences, Washington State University, Pullman, WA 99164-6420Jonathan D Soule - U.S. Department of Agriculture-Agricultural Research Service and Department of Crop and Soil Science and Graduate Program in Molecular Plant Sciences, Washington State University, Pullman, WA 99164-6420Karen M McGinnis - U.S. Department of Agriculture-Agricultural Research Service and Department of Crop and Soil Science and Graduate Program in Molecular Plant Sciences, Washington State University, Pullman, WA 99164-6420Camille M Steber - U.S. Department of Agriculture-Agricultural Research Service and Department of Crop and Soil Science and Graduate Program in Molecular Plant Sciences, Washington State University, Pullman, WA 99164-6420
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.101(34), pp.12771-12776
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- National Academy of Sciences
- Identifiers
- 99900547063001842
- Language
- English
- Resource Type
- Journal article