Journal article
Recognition of glycoprotein peroxidase via Con A-carrying self-assembly layer on gold
Biomacromolecules, Vol.8(7), pp.2142-2148
07/2007
Handle:
https://hdl.handle.net/2376/104097
PMID: 17569558
Abstract
We have successfully fabricated a self-assembled layer of concanavalin A (Con A) on a gold surface for recognition of glycoproteins. The type IV Con A is covalently bound to 11-mercaptoundecanoic acid (MUA) on gold with a 2-(5-norbornene-2,3-dicarboximido)-1,1,3,3-tetramethyluronium tetrafluoroborate (TNTU) linkage. The binding interaction between glycoproteins and self-assembled Con A is studied using horseradish peroxidase (HRP) as a model glycoprotein. Voltammetric, electrochemical impedance studies, and photometric activity measurements show the presence of both specific and nonspecific bindings of HRP to the Con A interface. The specific binding is attributed to the Con A-sugar interaction where Con A selectively recognizes the glycosylation sites of HRP. The catalytic current of the HRP-loaded electrode, because of catalytic oxidation of thionine in the presence of hydrogen peroxide (H2O2), is found to be proportional to the HRP concentrations in the incubation solution. A linear correlation coefficient of 0.993 was obtained over a wide HRP concentration range of 12.5 microg/mL to 1 mg/mL. The approach described in this study provides a simple yet selective means to immobilize glycoproteins on a solid support. The specific binding achieved is desirable in biosensor fabrication, glycoprotein separation, recognition, and purification as well as in drug-releasing systems.
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Details
- Title
- Recognition of glycoprotein peroxidase via Con A-carrying self-assembly layer on gold
- Creators
- Songqin Liu - College of Chemistry and Chemical Engineering, Southeast University, Nanjing, PR China. liusq@seu.edu.cnKewei WangDan DuYueming SunLin He
- Publication Details
- Biomacromolecules, Vol.8(7), pp.2142-2148
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- United States
- Identifiers
- 99900546831101842
- Language
- English
- Resource Type
- Journal article