Journal article
Recombinant vaccinia virus expression of Anaplasma marginale surface protein MSP-1a: effect of promoters, leader sequences and GPI anchor sequence on antibody response
Vaccine, Vol.12(5), pp.465-471
1994
Handle:
https://hdl.handle.net/2376/114120
PMID: 8023555
Abstract
Anaplasma marginale surface protein MSP-1a was expressed by recombinant vaccinia viruses with different promoters and as hybrid proteins. Transcription of msp1α with P11 late promoter resulted in more MSP-1a than with P7.5 early-late promoter; however, mice immunized with the recombinants had similar antibody titres. Recombinants expressing hybrid MSP-1a with either a murine leukaemia virus or a trypanosomal glycoprotein signal sequence did not enhance antibody responses and resulted in a diffuse intracellular distribution of MSP-1a which did not accumulate in the Golgi apparatus as was noted in the absence of these signal sequences. In contrast, antibody titres to MSP-1a in mice immunized with a recombinant virus expressing hybrid MSP-1a with a trypanosomal GPI anchor signal sequence were significantly increased over all other constructs.
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Details
- Title
- Recombinant vaccinia virus expression of Anaplasma marginale surface protein MSP-1a: effect of promoters, leader sequences and GPI anchor sequence on antibody response
- Creators
- Travis C McGuire - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAEdward B Stephens - Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USAGuy H Palmer - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USATerry F McElwain - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USACarol A Lichtensteiger - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USASteve R Leib - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAAnthony F Barbet - Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA
- Publication Details
- Vaccine, Vol.12(5), pp.465-471
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier Ltd
- Identifiers
- 99900548597901842
- Language
- English
- Resource Type
- Journal article