Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning

Irina Malinova; Hans-Henning Kunz; Saleh Alseekh; Karoline Herbst; Alisdair R Fernie; Markus Gierth; Joerg Fettke

doi:10.1371/journal.pone.0112468

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Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning

Journal article

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Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning

Irina Malinova, Hans-Henning Kunz, Saleh Alseekh, Karoline Herbst, Alisdair R Fernie, Markus Gierth and Joerg Fettke

PloS one, Vol.9(11), pp.e112468-e112468

2014

DOI: https://doi.org/10.1371/journal.pone.0112468

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https://hdl.handle.net/2376/101116

PMCID: PMC4234415

PMID: 25401493

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Abstract

Metabolomics

Arabidopsis - physiology

Plant Roots - metabolism

Seeds - metabolism

Isoenzymes

Carbohydrate Metabolism

Starch - metabolism

Metabolome

Phenotype

Plants, Genetically Modified

Cytosol - metabolism

Enzyme Activation

Phosphoglucomutase - genetics

Phosphoglucomutase - metabolism

Phosphoglucomutase (PGM) catalyses the interconversion of glucose 1-phosphate (G1P) and glucose 6-phosphate (G6P) and exists as plastidial (pPGM) and cytosolic (cPGM) isoforms. The plastidial isoform is essential for transitory starch synthesis in chloroplasts of leaves, whereas the cytosolic counterpart is essential for glucose phosphate partitioning and, therefore, for syntheses of sucrose and cell wall components. In Arabidopsis two cytosolic isoforms (PGM2 and PGM3) exist. Both PGM2 and PGM3 are redundant in function as single mutants reveal only small or no alterations compared to wild type with respect to plant primary metabolism. So far, there are no reports of Arabidopsis plants lacking the entire cPGM or total PGM activity, respectively. Therefore, amiRNA transgenic plants were generated and used for analyses of various parameters such as growth, development, and starch metabolism. The lack of the entire cPGM activity resulted in a strongly reduced growth revealed by decreased rosette fresh weight, shorter roots, and reduced seed production compared to wild type. By contrast content of starch, sucrose, maltose and cell wall components were significantly increased. The lack of both cPGM and pPGM activities in Arabidopsis resulted in dwarf growth, prematurely die off, and inability to develop a functional inflorescence. The combined results are discussed in comparison to potato, the only described mutant with lack of total PGM activity.

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Title: Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning
Creators: Irina Malinova - Plant Physiology, University of Potsdam, Potsdam-Golm, Germany; Biopolymers analytics, University of Potsdam, Potsdam-Golm, Germany
Hans-Henning Kunz - Plant Physiology, Washington State University, Pullman, Washington, United States of America; Department of Botany II, University of Cologne, Cologne, Germany
Saleh Alseekh - Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany
Karoline Herbst - Plant Physiology, University of Potsdam, Potsdam-Golm, Germany
Alisdair R Fernie - Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany
Markus Gierth - Department of Botany II, University of Cologne, Cologne, Germany
Joerg Fettke - Plant Physiology, University of Potsdam, Potsdam-Golm, Germany; Biopolymers analytics, University of Potsdam, Potsdam-Golm, Germany
Publication Details: PloS one, Vol.9(11), pp.e112468-e112468
Academic Unit: Biological Sciences, School of
Publisher: United States
Identifiers: 99900546613701842
Language: English
Resource Type: Journal article